Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana)

Bastian Franke, A.S. Jayasena, Mark F. Fisher, Joakim E. Swedberg, Nicolas L. Taylor, Joshua S. Mylne, K. Johan Rosengren

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new family of small plant peptides was recently described and found to be widespread throughout the Millereae and Heliantheae tribes of the sunflower family Asteraceae. These peptides originate from the post-translational processing of unusual seed-storage albumin genes, and have been termed PawS-derived peptides (PDPs). The prototypic family member is a 14-residue cyclic peptide with potent trypsin inhibitory activity named SunFlower Trypsin Inhibitor (SFTI-1). In this study we present the features of three new PDPs discovered in the seeds of the sunflower species Zinnia haageana by a combination of de novo transcriptomics and liquid chromatography-mass spectrometry. Two-dimensional solution NMR spectroscopy was used to elucidate their structural characteristics. All three Z. haageana peptides have well-defined folds with a head-to-tail cyclized peptide backbone and a single disulfide bond. Although two possess an anti-parallel β-sheet structure, like SFTI-1, the Z. haageana peptide PDP-21 has a more irregular backbone structure. Despite structural similarities with SFTI-1, PDP-20 was not able to inhibit trypsin, thus the functional roles of these peptides is yet to be discovered. Defining the structural features of the small cyclic peptides found in the sunflower family will be useful for guiding the exploitation of these peptides as scaffolds for grafting and protein engineering applications.

Original languageEnglish
Pages (from-to)806-817
Number of pages12
JournalBiopolymers
Volume106
Issue number6
DOIs
Publication statusPublished - 1 Nov 2016

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Cyclic Peptides
Peptides
Seed
Seeds
Helianthus
Trypsin
Protein Engineering
Asteraceae
Trypsin Inhibitors
Liquid chromatography
Scaffolds (biology)
Scaffolds
Liquid Chromatography
Disulfides
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Albumins
Mass Spectrometry
Magnetic Resonance Spectroscopy
Genes

Cite this

Franke, Bastian ; Jayasena, A.S. ; Fisher, Mark F. ; Swedberg, Joakim E. ; Taylor, Nicolas L. ; Mylne, Joshua S. ; Rosengren, K. Johan. / Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana). In: Biopolymers. 2016 ; Vol. 106, No. 6. pp. 806-817.
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Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana). / Franke, Bastian; Jayasena, A.S.; Fisher, Mark F.; Swedberg, Joakim E.; Taylor, Nicolas L.; Mylne, Joshua S.; Rosengren, K. Johan.

In: Biopolymers, Vol. 106, No. 6, 01.11.2016, p. 806-817.

Research output: Contribution to journalArticle

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AU - Franke, Bastian

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AU - Rosengren, K. Johan

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