Distribution of immunoreactive endothelin in the lungs of mice during respiratory viral infection

M.J. Carr, L.J. Spalding, Roy Goldie, Peter Henry

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Respiratory tract viral infections are associated with the generation of a wide array of pro-inflammatory cytokines, some of which enhance the release of the potent airway smooth muscle spasmogen, endothelin, from respiratory epithelial cells in tissue culture, The aim of this study was to determine whether the content and distribution of immunoreactive endothelin in the intact murine lung is increased during the course of a respiratory tract viral infection.Mice were inoculated intranasally with Influenza A/PR-8/34 virus or sterile vehicle and at various days postinoculation were sacrificed, and their lungs processed for either fluorescence immunohistochemistry with rabbit anti-endothelin sera or measurement of immunoreactive endothelin with an enzyme-linked immunosorbent assay (ELISA).At 2 and 4 days postinoculation, the content of immunoreactive endothelin in lung extracts of virus-infected mice was approximately twice that present in lung extracts from control mice (n=3-4, p
Original languageEnglish
Pages (from-to)79-85
JournalEuropean Respiratory Journal
Volume11
DOIs
Publication statusPublished - 1998

Fingerprint

Endothelins
Virus Diseases
Respiratory Tract Infections
Lung
Viruses
Human Influenza
Smooth Muscle
Fluorescence
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Cytokines
Rabbits
Serum

Cite this

@article{bc5daa287fab43adbf53a161c407e040,
title = "Distribution of immunoreactive endothelin in the lungs of mice during respiratory viral infection",
abstract = "Respiratory tract viral infections are associated with the generation of a wide array of pro-inflammatory cytokines, some of which enhance the release of the potent airway smooth muscle spasmogen, endothelin, from respiratory epithelial cells in tissue culture, The aim of this study was to determine whether the content and distribution of immunoreactive endothelin in the intact murine lung is increased during the course of a respiratory tract viral infection.Mice were inoculated intranasally with Influenza A/PR-8/34 virus or sterile vehicle and at various days postinoculation were sacrificed, and their lungs processed for either fluorescence immunohistochemistry with rabbit anti-endothelin sera or measurement of immunoreactive endothelin with an enzyme-linked immunosorbent assay (ELISA).At 2 and 4 days postinoculation, the content of immunoreactive endothelin in lung extracts of virus-infected mice was approximately twice that present in lung extracts from control mice (n=3-4, p",
author = "M.J. Carr and L.J. Spalding and Roy Goldie and Peter Henry",
year = "1998",
doi = "10.1183/09031936.98.11010079",
language = "English",
volume = "11",
pages = "79--85",
journal = "The European Respiratory Journal",
issn = "0903-1936",
publisher = "European Respiratory Soc Journals Ltd",

}

Distribution of immunoreactive endothelin in the lungs of mice during respiratory viral infection. / Carr, M.J.; Spalding, L.J.; Goldie, Roy; Henry, Peter.

In: European Respiratory Journal, Vol. 11, 1998, p. 79-85.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Distribution of immunoreactive endothelin in the lungs of mice during respiratory viral infection

AU - Carr, M.J.

AU - Spalding, L.J.

AU - Goldie, Roy

AU - Henry, Peter

PY - 1998

Y1 - 1998

N2 - Respiratory tract viral infections are associated with the generation of a wide array of pro-inflammatory cytokines, some of which enhance the release of the potent airway smooth muscle spasmogen, endothelin, from respiratory epithelial cells in tissue culture, The aim of this study was to determine whether the content and distribution of immunoreactive endothelin in the intact murine lung is increased during the course of a respiratory tract viral infection.Mice were inoculated intranasally with Influenza A/PR-8/34 virus or sterile vehicle and at various days postinoculation were sacrificed, and their lungs processed for either fluorescence immunohistochemistry with rabbit anti-endothelin sera or measurement of immunoreactive endothelin with an enzyme-linked immunosorbent assay (ELISA).At 2 and 4 days postinoculation, the content of immunoreactive endothelin in lung extracts of virus-infected mice was approximately twice that present in lung extracts from control mice (n=3-4, p

AB - Respiratory tract viral infections are associated with the generation of a wide array of pro-inflammatory cytokines, some of which enhance the release of the potent airway smooth muscle spasmogen, endothelin, from respiratory epithelial cells in tissue culture, The aim of this study was to determine whether the content and distribution of immunoreactive endothelin in the intact murine lung is increased during the course of a respiratory tract viral infection.Mice were inoculated intranasally with Influenza A/PR-8/34 virus or sterile vehicle and at various days postinoculation were sacrificed, and their lungs processed for either fluorescence immunohistochemistry with rabbit anti-endothelin sera or measurement of immunoreactive endothelin with an enzyme-linked immunosorbent assay (ELISA).At 2 and 4 days postinoculation, the content of immunoreactive endothelin in lung extracts of virus-infected mice was approximately twice that present in lung extracts from control mice (n=3-4, p

U2 - 10.1183/09031936.98.11010079

DO - 10.1183/09031936.98.11010079

M3 - Article

VL - 11

SP - 79

EP - 85

JO - The European Respiratory Journal

JF - The European Respiratory Journal

SN - 0903-1936

ER -