Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study

ENDIA Study Grp; Ki Wook Kim; Digby W. Allen; Thomas Briese; Jennifer J. Couper; Simon C. Barry; Peter G. Colman; Andrew M. Cotterill; Elizabeth A. Davis; Lynne C. Giles; Leonard C. Harrison; Mark Harris; Aveni Haynes; Jessica L. Horton; Sonia R. Isaacs; Komal Jain; Walter Ian Lipkin; Grant Morahan; Claire Morbey; Ignatius C. N. Pang; Anthony T. Papenfuss; Megan A. S. Penno; Richard O. Sinnott; Georgia Soldatos; Rebecca L. Thomson; Peter J. Vuillermin; John M. Wentworth; Marc R. Wilkins; William D. Rawlinson; Maria E. Craig

doi:10.1093/ofid/ofz025

Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study

ENDIA Study Grp, Ki Wook Kim, Digby W. Allen, Thomas Briese, Jennifer J. Couper, Simon C. Barry, Peter G. Colman, Andrew M. Cotterill, Elizabeth A. Davis, Lynne C. Giles, Leonard C. Harrison, Mark Harris, Aveni Haynes, Jessica L. Horton, Sonia R. Isaacs, Komal Jain, Walter Ian Lipkin, Grant Morahan, Claire Morbey, Ignatius C. N. PangAnthony T. Papenfuss, Megan A. S. Penno, Richard O. Sinnott, Georgia Soldatos, Rebecca L. Thomson, Peter J. Vuillermin, John M. Wentworth, Marc R. Wilkins, William D. Rawlinson, Maria E. Craig

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Abstract

Background. The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.

Methods. In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared between women with and without T1D.

Results. Two viruses were more prevalent in pregnant women with T1D: picobirnaviruses (odds ratio [OR], 4.2; 95% confidence interval [CI], 1.0-17.1; P =.046) and tobamoviruses (OR, 3.2; 95% CI, 1.1-9.3; P =.037). The abundance of 77 viruses significantly differed between the 2 maternal groups (>= 2-fold difference; P

Conclusions. These findings provide novel insight into the composition of the gut virome during pregnancy and demonstrate a distinct profile of viruses in women with T1D.

Original language	English
Article number	025
Number of pages	8
Journal	Open Forum Infectious Diseases
Volume	6
Issue number	2
DOIs	https://doi.org/10.1093/ofid/ofz025
Publication status	Published - Feb 2019

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10.1093/ofid/ofz025Licence: CC BY-NC-ND

Centre of Research Excellence for the Protection of Pancreatic Beta Cells
Couper, J. (Investigator 01), Harrison, L. (Investigator 02), Craig, M. (Investigator 03), Morahan, G. (Investigator 04), Colman, P. (Investigator 05), Wentworth, J. (Investigator 06), Papenfuss, A. (Investigator 07) & Rawlinson, W. (Investigator 08)
NHMRC National Health and Medical Research Council
1/12/14 → 30/11/19
Project: Research

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ENDIA Study Grp, Kim, K. W., Allen, D. W., Briese, T., Couper, J. J., Barry, S. C., Colman, P. G., Cotterill, A. M., Davis, E. A., Giles, L. C., Harrison, L. C., Harris, M., Haynes, A., Horton, J. L., Isaacs, S. R., Jain, K., Lipkin, W. I., Morahan, G., Morbey, C., ... Craig, M. E. (2019). Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study. Open Forum Infectious Diseases, 6(2), Article 025. https://doi.org/10.1093/ofid/ofz025

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title = "Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study",

abstract = "Background. The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.Methods. In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared between women with and without T1D.Results. Two viruses were more prevalent in pregnant women with T1D: picobirnaviruses (odds ratio [OR], 4.2; 95% confidence interval [CI], 1.0-17.1; P =.046) and tobamoviruses (OR, 3.2; 95% CI, 1.1-9.3; P =.037). The abundance of 77 viruses significantly differed between the 2 maternal groups (>= 2-fold difference; PConclusions. These findings provide novel insight into the composition of the gut virome during pregnancy and demonstrate a distinct profile of viruses in women with T1D.",

keywords = "enterovirus, pregnancy, type 1 diabetes, virome capture sequencing, BLOOD MONONUCLEAR-CELLS, COXSACKIEVIRUS B1, ENTEROVIRUS RNA, VIRUS, INFECTION, METAGENOMICS, AUTOIMMUNITY, CHILDREN, ONSET",

author = "{ENDIA Study Grp} and Kim, {Ki Wook} and Allen, {Digby W.} and Thomas Briese and Couper, {Jennifer J.} and Barry, {Simon C.} and Colman, {Peter G.} and Cotterill, {Andrew M.} and Davis, {Elizabeth A.} and Giles, {Lynne C.} and Harrison, {Leonard C.} and Mark Harris and Aveni Haynes and Horton, {Jessica L.} and Isaacs, {Sonia R.} and Komal Jain and Lipkin, {Walter Ian} and Grant Morahan and Claire Morbey and Pang, {Ignatius C. N.} and Papenfuss, {Anthony T.} and Penno, {Megan A. S.} and Sinnott, {Richard O.} and Georgia Soldatos and Thomson, {Rebecca L.} and Vuillermin, {Peter J.} and Wentworth, {John M.} and Wilkins, {Marc R.} and Rawlinson, {William D.} and Craig, {Maria E.}",

year = "2019",

month = feb,

doi = "10.1093/ofid/ofz025",

language = "English",

volume = "6",

journal = "Open Forum Infectious Diseases",

issn = "2328-8957",

publisher = "Oxford University Press",

number = "2",

}

ENDIA Study Grp, Kim, KW, Allen, DW, Briese, T, Couper, JJ, Barry, SC, Colman, PG, Cotterill, AM, Davis, EA, Giles, LC, Harrison, LC, Harris, M, Haynes, A, Horton, JL, Isaacs, SR, Jain, K, Lipkin, WI, Morahan, G, Morbey, C, Pang, ICN, Papenfuss, AT, Penno, MAS, Sinnott, RO, Soldatos, G, Thomson, RL, Vuillermin, PJ, Wentworth, JM, Wilkins, MR, Rawlinson, WD & Craig, ME 2019, 'Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study', Open Forum Infectious Diseases, vol. 6, no. 2, 025. https://doi.org/10.1093/ofid/ofz025

TY - JOUR

T1 - Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study

AU - ENDIA Study Grp

AU - Kim, Ki Wook

AU - Allen, Digby W.

AU - Briese, Thomas

AU - Couper, Jennifer J.

AU - Barry, Simon C.

AU - Colman, Peter G.

AU - Cotterill, Andrew M.

AU - Davis, Elizabeth A.

AU - Giles, Lynne C.

AU - Harrison, Leonard C.

AU - Harris, Mark

AU - Haynes, Aveni

AU - Horton, Jessica L.

AU - Isaacs, Sonia R.

AU - Jain, Komal

AU - Lipkin, Walter Ian

AU - Morahan, Grant

AU - Morbey, Claire

AU - Pang, Ignatius C. N.

AU - Papenfuss, Anthony T.

AU - Penno, Megan A. S.

AU - Sinnott, Richard O.

AU - Soldatos, Georgia

AU - Thomson, Rebecca L.

AU - Vuillermin, Peter J.

AU - Wentworth, John M.

AU - Wilkins, Marc R.

AU - Rawlinson, William D.

AU - Craig, Maria E.

PY - 2019/2

Y1 - 2019/2

N2 - Background. The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.Methods. In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared between women with and without T1D.Results. Two viruses were more prevalent in pregnant women with T1D: picobirnaviruses (odds ratio [OR], 4.2; 95% confidence interval [CI], 1.0-17.1; P =.046) and tobamoviruses (OR, 3.2; 95% CI, 1.1-9.3; P =.037). The abundance of 77 viruses significantly differed between the 2 maternal groups (>= 2-fold difference; PConclusions. These findings provide novel insight into the composition of the gut virome during pregnancy and demonstrate a distinct profile of viruses in women with T1D.

AB - Background. The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.Methods. In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared between women with and without T1D.Results. Two viruses were more prevalent in pregnant women with T1D: picobirnaviruses (odds ratio [OR], 4.2; 95% confidence interval [CI], 1.0-17.1; P =.046) and tobamoviruses (OR, 3.2; 95% CI, 1.1-9.3; P =.037). The abundance of 77 viruses significantly differed between the 2 maternal groups (>= 2-fold difference; PConclusions. These findings provide novel insight into the composition of the gut virome during pregnancy and demonstrate a distinct profile of viruses in women with T1D.

KW - enterovirus

KW - pregnancy

KW - type 1 diabetes

KW - virome capture sequencing

KW - BLOOD MONONUCLEAR-CELLS

KW - COXSACKIEVIRUS B1

KW - ENTEROVIRUS RNA

KW - VIRUS

KW - INFECTION

KW - METAGENOMICS

KW - AUTOIMMUNITY

KW - CHILDREN

KW - ONSET

U2 - 10.1093/ofid/ofz025

DO - 10.1093/ofid/ofz025

M3 - Article

C2 - 30815502

SN - 2328-8957

VL - 6

JO - Open Forum Infectious Diseases

JF - Open Forum Infectious Diseases

IS - 2

M1 - 025

ER -

Distinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study

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