Dissecting the molecular mode of action of Restorer-of-fertility-like proteins in plant mitochondria

Sang Dang Huynh

doi:10.26182/1n26-kj88

Dissecting the molecular mode of action of Restorer-of-fertility-like proteins in plant mitochondria

Sang Dang Huynh

School of Molecular Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

The molecular mode of interactions between restorer-of-fertility (Rf) proteins and transcripts causing cytoplasmic male sterility (CMS) in plants remains unknown. I discovered a unique sequence feature, RfCTD, in Rfs and their homologous, restorer-of-fertility-like (RFL) proteins. The proportions of RFLscorrelated to reproductive strategies. AlphaFold predictions infer domain poses a distinct function. Truncation experiments of A. thaliana RNA PROCESSING FACTOR 2 (RPF2) showed the importance of RfCTD. The domain cannot cleave the RNA target on its own. Immunoprecipitation and yeast interactome assay did not find promising partners with nucleolytic activity. The findings have expanded our understanding of Rf and RFL proteins.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Melonek, Joanna, Supervisor Small, Ian, Supervisor Millar, Harvey, Supervisor Colas des Francs-Small, Catherine, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	4 Sept 2023
DOIs	https://doi.org/10.26182/1n26-kj88
Publication status	Unpublished - 2023

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10.26182/1n26-kj88

THESIS - DOCTOR OF PHILOSOPHY - HUYNH Sang Dang - 2023
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A unique C-terminal domain contributes to the molecular function of Restorer-of-fertility proteins in plant mitochondria
Huynh, S. D., Melonek, J., Colas des Francs-Small, C., Bond, C. S. & Small, I., Oct 2023, In: The New Phytologist. 240, 2, p. 830-845 16 p.
Research output: Contribution to journal › Article › peer-review

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title = "Dissecting the molecular mode of action of Restorer-of-fertility-like proteins in plant mitochondria",

abstract = "The molecular mode of interactions between restorer-of-fertility (Rf) proteins and transcripts causing cytoplasmic male sterility (CMS) in plants remains unknown. I discovered a unique sequence feature, RfCTD, in Rfs and their homologous, restorer-of-fertility-like (RFL) proteins. The proportions of RFLscorrelated to reproductive strategies. AlphaFold predictions infer domain poses a distinct function. Truncation experiments of A. thaliana RNA PROCESSING FACTOR 2 (RPF2) showed the importance of RfCTD. The domain cannot cleave the RNA target on its own. Immunoprecipitation and yeast interactome assay did not find promising partners with nucleolytic activity. The findings have expanded our understanding of Rf and RFL proteins.",

keywords = "restorer-of-fertility-like (RFL) proteins, cytoplasmic male sterility (CMS), RfCTD",

author = "Huynh, \{Sang Dang\}",

year = "2023",

doi = "10.26182/1n26-kj88",

language = "English",

school = "The University of Western Australia",

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AU - Huynh, Sang Dang

PY - 2023

Y1 - 2023

N2 - The molecular mode of interactions between restorer-of-fertility (Rf) proteins and transcripts causing cytoplasmic male sterility (CMS) in plants remains unknown. I discovered a unique sequence feature, RfCTD, in Rfs and their homologous, restorer-of-fertility-like (RFL) proteins. The proportions of RFLscorrelated to reproductive strategies. AlphaFold predictions infer domain poses a distinct function. Truncation experiments of A. thaliana RNA PROCESSING FACTOR 2 (RPF2) showed the importance of RfCTD. The domain cannot cleave the RNA target on its own. Immunoprecipitation and yeast interactome assay did not find promising partners with nucleolytic activity. The findings have expanded our understanding of Rf and RFL proteins.

AB - The molecular mode of interactions between restorer-of-fertility (Rf) proteins and transcripts causing cytoplasmic male sterility (CMS) in plants remains unknown. I discovered a unique sequence feature, RfCTD, in Rfs and their homologous, restorer-of-fertility-like (RFL) proteins. The proportions of RFLscorrelated to reproductive strategies. AlphaFold predictions infer domain poses a distinct function. Truncation experiments of A. thaliana RNA PROCESSING FACTOR 2 (RPF2) showed the importance of RfCTD. The domain cannot cleave the RNA target on its own. Immunoprecipitation and yeast interactome assay did not find promising partners with nucleolytic activity. The findings have expanded our understanding of Rf and RFL proteins.

KW - restorer-of-fertility-like (RFL) proteins

KW - cytoplasmic male sterility (CMS)

KW - RfCTD

U2 - 10.26182/1n26-kj88

DO - 10.26182/1n26-kj88

M3 - Doctoral Thesis

ER -

Dissecting the molecular mode of action of Restorer-of-fertility-like proteins in plant mitochondria

Abstract

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Research output

A unique C-terminal domain contributes to the molecular function of Restorer-of-fertility proteins in plant mitochondria

Cite this