Dissecting the molecular mode of action of Restorer-of-fertility-like proteins in plant mitochondria

Research output: ThesisDoctoral Thesis

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Abstract

The molecular mode of interactions between restorer-of-fertility (Rf) proteins and transcripts causing cytoplasmic male sterility (CMS) in plants remains unknown. I discovered a unique sequence feature, RfCTD, in Rfs and their homologous, restorer-of-fertility-like (RFL) proteins. The proportions of RFLscorrelated to reproductive strategies. AlphaFold predictions infer domain poses a distinct function. Truncation experiments of A. thaliana RNA PROCESSING FACTOR 2 (RPF2) showed the importance of RfCTD. The domain cannot cleave the RNA target on its own. Immunoprecipitation and yeast interactome assay did not find promising partners with nucleolytic activity. The findings have expanded our understanding of Rf and RFL proteins.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Melonek, Joanna, Supervisor
  • Small, Ian, Supervisor
  • Millar, Harvey, Supervisor
  • Colas des Francs-Small, Catherine, Supervisor
Thesis sponsors
Award date4 Sept 2023
DOIs
Publication statusUnpublished - 2023

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