Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains. © 2013 American Chemical Society.
Yin, W. B., Chooi, H., Smith, A. R., Cacho, R. A., Hu, Y., White, T. C., & Tang, Y. (2013). Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans. ACS Synthetic Biology, 2(11), 629-634. https://doi.org/10.1021/sb400048b