Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans

W.B. Yin, Heng Chooi, A.R. Smith, R.A. Cacho, Y. Hu, T.C. White, Y. Tang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains. © 2013 American Chemical Society.
Original languageEnglish
Pages (from-to)629-634
JournalACS Synthetic Biology
Volume2
Issue number11
DOIs
Publication statusPublished - 2013

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Polyketides
Aspergillus nidulans
Arthrodermataceae
Aspergillus
Metabolites
Genes
Cloning
Immunosuppressive Agents
Yeast
Skin
Trichophyton
Animals
Aspergillus fumigatus
Multigene Family
Genetic Recombination
Organism Cloning
Yeasts
Genome
Infection

Cite this

Yin, W.B. ; Chooi, Heng ; Smith, A.R. ; Cacho, R.A. ; Hu, Y. ; White, T.C. ; Tang, Y. / Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans. In: ACS Synthetic Biology. 2013 ; Vol. 2, No. 11. pp. 629-634.
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Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans. / Yin, W.B.; Chooi, Heng; Smith, A.R.; Cacho, R.A.; Hu, Y.; White, T.C.; Tang, Y.

In: ACS Synthetic Biology, Vol. 2, No. 11, 2013, p. 629-634.

Research output: Contribution to journalArticle

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