Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans

W.B. Yin, Heng Chooi, A.R. Smith, R.A. Cacho, Y. Hu, T.C. White, Y. Tang

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains. © 2013 American Chemical Society.
Original languageEnglish
Pages (from-to)629-634
JournalACS Synthetic Biology
Volume2
Issue number11
DOIs
Publication statusPublished - 2013

Fingerprint Dive into the research topics of 'Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in aspergillus nidulans'. Together they form a unique fingerprint.

  • Cite this