Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia

J. Hobson-Peters; D. Warrilow; B.J. Mclean; D. Watterson; A.M.G. Colmant; A.F. Van Den Hurk; S. Hall-Mendelin; M.L. Hastie; J.J. Gorman; J.J. Harrison; N.A. Prow; R.T. Barnard; Richard Allcock; C.A. Johansen; R.A. Hall

doi:10.1016/j.virol.2015.11.003

Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia

J. Hobson-Peters, D. Warrilow, B.J. Mclean, D. Watterson, A.M.G. Colmant, A.F. Van Den Hurk, S. Hall-Mendelin, M.L. Hastie, J.J. Gorman, J.J. Harrison, N.A. Prow, R.T. Barnard, Richard Allcock, C.A. Johansen, R.A. Hall

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

© 2016 Elsevier Inc. Insect-specific viruses belonging to significant arboviral families have recently been discovered. These viruses appear to be maintained within the insect population without the requirement for replication in a vertebrate host. Mosquitoes collected from Badu Island in the Torres Strait in 2003 were analysed for insect-specific viruses. A novel bunyavirus was isolated in high prevalence from Culex spp. The new virus, provisionally called Badu virus (BADUV), replicated in mosquito cells of both Culex and Aedes origin, but failed to replicate in vertebrate cells. Genomic sequencing revealed that the virus was distinct from sequenced bunyavirus isolates reported to date, but phylogenetically clustered most closely with recently discovered mosquito-borne, insect-specific bunyaviruses in the newly proposed Goukovirus genus. The detection of a functional furin cleavage motif upstream of the two glycoproteins in the M segment-encoded polyprotein suggests that BADUV may employ a unique strategy to process the virion glycoproteins.

Original language	English
Pages (from-to)	269-281
Number of pages	13
Journal	Virology
Volume	489
DOIs	https://doi.org/10.1016/j.virol.2015.11.003
Publication status	Published - 1 Feb 2016

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Hobson-Peters, J., Warrilow, D., Mclean, B. J., Watterson, D., Colmant, A. M. G., Van Den Hurk, A. F., Hall-Mendelin, S., Hastie, M. L., Gorman, J. J., Harrison, J. J., Prow, N. A., Barnard, R. T., Allcock, R., Johansen, C. A., & Hall, R. A. (2016). Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia. Virology, 489, 269-281. https://doi.org/10.1016/j.virol.2015.11.003

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title = "Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia",

abstract = "{\textcopyright} 2016 Elsevier Inc. Insect-specific viruses belonging to significant arboviral families have recently been discovered. These viruses appear to be maintained within the insect population without the requirement for replication in a vertebrate host. Mosquitoes collected from Badu Island in the Torres Strait in 2003 were analysed for insect-specific viruses. A novel bunyavirus was isolated in high prevalence from Culex spp. The new virus, provisionally called Badu virus (BADUV), replicated in mosquito cells of both Culex and Aedes origin, but failed to replicate in vertebrate cells. Genomic sequencing revealed that the virus was distinct from sequenced bunyavirus isolates reported to date, but phylogenetically clustered most closely with recently discovered mosquito-borne, insect-specific bunyaviruses in the newly proposed Goukovirus genus. The detection of a functional furin cleavage motif upstream of the two glycoproteins in the M segment-encoded polyprotein suggests that BADUV may employ a unique strategy to process the virion glycoproteins.",

author = "J. Hobson-Peters and D. Warrilow and B.J. Mclean and D. Watterson and A.M.G. Colmant and {Van Den Hurk}, A.F. and S. Hall-Mendelin and M.L. Hastie and J.J. Gorman and J.J. Harrison and N.A. Prow and R.T. Barnard and Richard Allcock and C.A. Johansen and R.A. Hall",

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Hobson-Peters, J, Warrilow, D, Mclean, BJ, Watterson, D, Colmant, AMG, Van Den Hurk, AF, Hall-Mendelin, S, Hastie, ML, Gorman, JJ, Harrison, JJ, Prow, NA, Barnard, RT, Allcock, R, Johansen, CA & Hall, RA 2016, 'Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia', Virology, vol. 489, pp. 269-281. https://doi.org/10.1016/j.virol.2015.11.003

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T1 - Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia

AU - Hobson-Peters, J.

AU - Warrilow, D.

AU - Mclean, B.J.

AU - Watterson, D.

AU - Colmant, A.M.G.

AU - Van Den Hurk, A.F.

AU - Hall-Mendelin, S.

AU - Hastie, M.L.

AU - Gorman, J.J.

AU - Harrison, J.J.

AU - Prow, N.A.

AU - Barnard, R.T.

AU - Allcock, Richard

AU - Johansen, C.A.

AU - Hall, R.A.

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Y1 - 2016/2/1

N2 - © 2016 Elsevier Inc. Insect-specific viruses belonging to significant arboviral families have recently been discovered. These viruses appear to be maintained within the insect population without the requirement for replication in a vertebrate host. Mosquitoes collected from Badu Island in the Torres Strait in 2003 were analysed for insect-specific viruses. A novel bunyavirus was isolated in high prevalence from Culex spp. The new virus, provisionally called Badu virus (BADUV), replicated in mosquito cells of both Culex and Aedes origin, but failed to replicate in vertebrate cells. Genomic sequencing revealed that the virus was distinct from sequenced bunyavirus isolates reported to date, but phylogenetically clustered most closely with recently discovered mosquito-borne, insect-specific bunyaviruses in the newly proposed Goukovirus genus. The detection of a functional furin cleavage motif upstream of the two glycoproteins in the M segment-encoded polyprotein suggests that BADUV may employ a unique strategy to process the virion glycoproteins.

AB - © 2016 Elsevier Inc. Insect-specific viruses belonging to significant arboviral families have recently been discovered. These viruses appear to be maintained within the insect population without the requirement for replication in a vertebrate host. Mosquitoes collected from Badu Island in the Torres Strait in 2003 were analysed for insect-specific viruses. A novel bunyavirus was isolated in high prevalence from Culex spp. The new virus, provisionally called Badu virus (BADUV), replicated in mosquito cells of both Culex and Aedes origin, but failed to replicate in vertebrate cells. Genomic sequencing revealed that the virus was distinct from sequenced bunyavirus isolates reported to date, but phylogenetically clustered most closely with recently discovered mosquito-borne, insect-specific bunyaviruses in the newly proposed Goukovirus genus. The detection of a functional furin cleavage motif upstream of the two glycoproteins in the M segment-encoded polyprotein suggests that BADUV may employ a unique strategy to process the virion glycoproteins.

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DO - 10.1016/j.virol.2015.11.003

M3 - Article

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VL - 489

SP - 269

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JO - Virology

JF - Virology

SN - 0042-6822

ER -

Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia

Abstract

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