Directed neutrophil migration to IL-8 is increased in cystic fibrosis: a study of the effect of erythromycin

Siobhan Brennan, D. Cooper, Peter Sly

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background-The aim of this study was to compare neutrophil migration in cystic fibrosis (CF) and non-CF populations and to investigate the effect of erythromycin on directed migration of neutrophils (PMNs) in CF.Methods-PMNs were isolated and their migratory capacity in response to interleukin-8 (IL-8) or f-Met-Leu-Phe (fMLP) in the presence or absence of erythromycin (1-100 mug/ml) was assessed.Results-CF derived PMNs showed significantly increased migration to IL-8 but not to fMLP compared with non-CF PMNs. Erythromycin had no significant effect on migration responses to IL-8 and in vitro exposure of PMNs to erythromycin had no effect.Conclusions-CF derived PMNs show higher migratory responsiveness to IL-8 but not to fMLP, suggesting that CF PMNs may be "primed" to IL-8 which is significantly increased in CF serum compared with non-CF serum. Treatment with erythromycin had no significant effect on PMN migration in vitro.
Original languageEnglish
Pages (from-to)62-64
JournalThorax
Volume56
DOIs
Publication statusPublished - 2001

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