Direct evidence that ganglioside is an integral component of the thyrotropin receptor

W. Kielczynski, L. C. Harrison, P. J. Leedman

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30 Citations (Scopus)


Gangliosides were extracted from purified human and porcine thyrotropin (TSH) receptors (TSH-R) and were detected by probing with an 125I-labeled sialic acid-specific lectin, Limax flavus agglutinin. Gangliosides copurified with human and porcine TSH-R migrated between monosialoganglioside GM1 and disialoganglioside GD1a. Ceramide glycanase digestion of the purified human TSH-R-associated glycolipid confirmed its ganglioside nature. It was resistant to Vibrio cholerae sialidase, which digests all gangliosides except GM1, but was sensitive to Arthrobacter ureafaciens sialidase, which digests all gangliosides including GM1. These findings indicate that the human TSH-R contains ganglioside that belongs to the galactosyl(β1 → 3)-N-acetylgalactosaminyl(β1 → 4)-[N-acetylneuraminyl(α2 → 3)]galactosyl(β1 → 4)glucosyl(β1 → 1)ceramide (GM1) family. Its intimate association with receptor protein role for ganglioside in the structure and function of the TSH-R.

Original languageEnglish
Pages (from-to)1991-1995
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number5
Publication statusPublished - 1991
Externally publishedYes


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