Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

S. Ohshima, S. Mori, A. Shigenari, A. Miyamoto, M. Takasu, N. Imaeda, S. Nunomura, Y. Okayama, M. Tanaka, H. Kitagawa, Jerzy Kulski, H. Inoko, A. Ando, Y. Kametani

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells with a likely role in cell survival, proliferation and differentiation. In order to study the differentiation activity of porcine CD117 hematopoietic cells in vitro and in vivo we prepared an anti-swine CD117 Mab (2A1) with high specificity for flow-cytometrical analysis. The 2A1 Mab did not recognize mouse or human mast cells suggesting that 2A1 is species-specific. Swine bone marrow (BM) CD117+ cells differentiated in vitro mainly into erythroid and monocyte lineages in the methylcellulose-based colony assay. When the swine BM CD117+ cells were transplanted in vivo into immunodeficient NOG (NOD/SCID/IL-2γcnull) mice, a significant amount of swine CD45+ leukocytes, including CD3 positive T cells, were developed in the mice. These results revealed that the swine BM CD117+ cells possess hematopoietic stem/progenitor activity and when monitored in immunodeficient mice or in vitro they can develop into lymphoid, erythroid, and myeloid cells efficiently with the new monoclonal antibody.
    Original languageEnglish
    Pages (from-to)308-315
    JournalBioScience Trends
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - 2014

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