Differential proteomics reveals novel insights into Nosema–honey bee interactions

Christoph Kurze, Ryan Dosselli, Julia Grassl, Yves Le Conte, Per Kryger, Boris Baer, Robin F A Moritz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Host manipulation is a common strategy by parasites to reduce host defense responses, enhance development, host exploitation, reproduction and, ultimately, transmission success. As these parasitic modifications can reduce host fitness, increased selection pressure may result in reciprocal adaptations of the host. Whereas the majority of studies on host manipulation have explored resistance against parasites (i.e. ability to prevent or limit an infection), data describing tolerance mechanisms (i.e. ability to limit harm of an infection) are scarce. By comparing differential protein abundance, we provide evidence of host-parasite interactions in the midgut proteomes of N. ceranae-infected and uninfected honey bees from both Nosema-tolerant and Nosema-sensitive lineages. We identified 16 proteins out of 661 protein spots that were differentially abundant between experimental groups. In general, infections of Nosema resulted in an up-regulation of the bee's energy metabolism. Additionally, we identified 8 proteins that were differentially abundant between tolerant and sensitive honey bees regardless of the Nosema infection. Those proteins were linked to metabolism, response to oxidative stress and apoptosis. In addition to bee proteins, we also identified 3 Nosema ceranae proteins. Interestingly, abundance of two of these Nosema proteins were significantly higher in infected Nosema-sensitive honeybees relative to the infected Nosema-tolerant lineage. This may provide a novel candidate for studying the molecular interplay between N. ceranae and its honey bee host in more detail.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume79
DOIs
Publication statusPublished - 1 Dec 2016

Fingerprint

Nosema
Bees
Proteomics
proteomics
Apoidea
honey bees
Honey
Proteins
proteins
Infection
infection
Parasites
Nosema ceranae
parasites
Host-Parasite Interactions
Oxidative stress
host-parasite relationships
Proteome
proteome
Metabolism

Cite this

Kurze, Christoph ; Dosselli, Ryan ; Grassl, Julia ; Le Conte, Yves ; Kryger, Per ; Baer, Boris ; Moritz, Robin F A. / Differential proteomics reveals novel insights into Nosema–honey bee interactions. In: Insect Biochemistry and Molecular Biology. 2016 ; Vol. 79. pp. 42-49.
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Differential proteomics reveals novel insights into Nosema–honey bee interactions. / Kurze, Christoph; Dosselli, Ryan; Grassl, Julia; Le Conte, Yves; Kryger, Per; Baer, Boris; Moritz, Robin F A.

In: Insect Biochemistry and Molecular Biology, Vol. 79, 01.12.2016, p. 42-49.

Research output: Contribution to journalArticle

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AU - Moritz, Robin F A

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