Differential gene expression analysis in early and late erythroid progenitor cells in β-thalassaemia: an in vitro comparative study between normal and β-Thalassaemia erythroblasts

Luke Benjamin Forster

    Research output: ThesisDoctoral Thesis

    285 Downloads (Pure)

    Abstract

    This study examines erythropoiesis in B-thalassaemia major in a laboratory setting. A gene expression study demonstrated dysregulation of 384 genes in B-thalassaemia erythroblasts cultured for 14 days. An expected role for apoptosis and the cell cycle was however not clear at this stage and as such functional studies were performed which demonstrated no difference in apoptosis and a decrease in the proportion of cells in M-phase. Downregulation of the NF-KB pathway was demonstrated and as such efforts were made to inhibit this pathway in erythroblasts in vitro. This led to the discovery that RNF-187 is regulated by this pathway.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    Supervisors/Advisors
    • Ghassemifar, Reza, Supervisor
    • Erber, Wendy, Supervisor
    • Finlayson, Jill, Supervisor
    Award date13 Jul 2016
    Publication statusUnpublished - 2016

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