Differential gene expression analysis in early and late erythroid progenitor cells in β-thalassaemia: an in vitro comparative study between normal and β-Thalassaemia erythroblasts

Luke Benjamin Forster

Differential gene expression analysis in early and late erythroid progenitor cells in β-thalassaemia: an in vitro comparative study between normal and β-Thalassaemia erythroblasts

Luke Benjamin Forster

Research output: Thesis › Doctoral Thesis

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Abstract

This study examines erythropoiesis in B-thalassaemia major in a laboratory setting. A gene expression study demonstrated dysregulation of 384 genes in B-thalassaemia erythroblasts cultured for 14 days. An expected role for apoptosis and the cell cycle was however not clear at this stage and as such functional studies were performed which demonstrated no difference in apoptosis and a decrease in the proportion of cells in M-phase. Downregulation of the NF-KB pathway was demonstrated and as such efforts were made to inhibit this pathway in erythroblasts in vitro. This led to the discovery that RNF-187 is regulated by this pathway.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Ghassemifar, Reza, Supervisor Erber, Wendy, Supervisor Finlayson, Jill, Supervisor
Award date	13 Jul 2016
Publication status	Unpublished - 2016

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THESIS - DOCTOR OF PHILOSOPHY - FORSTER Luke Benjamin - 2016
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title = "Differential gene expression analysis in early and late erythroid progenitor cells in β-thalassaemia: an in vitro comparative study between normal and β-Thalassaemia erythroblasts",

abstract = "This study examines erythropoiesis in B-thalassaemia major in a laboratory setting. A gene expression study demonstrated dysregulation of 384 genes in B-thalassaemia erythroblasts cultured for 14 days. An expected role for apoptosis and the cell cycle was however not clear at this stage and as such functional studies were performed which demonstrated no difference in apoptosis and a decrease in the proportion of cells in M-phase. Downregulation of the NF-KB pathway was demonstrated and as such efforts were made to inhibit this pathway in erythroblasts in vitro. This led to the discovery that RNF-187 is regulated by this pathway.",

keywords = "B-thalassaemia, Microarray, Quantitative real-time PCR, Gene expression, Erythropoiesis, Apoptosis, Cell cycle, Proliferation",

author = "Forster, {Luke Benjamin}",

year = "2016",

language = "English",

school = "The University of Western Australia",

}

TY - BOOK

T1 - Differential gene expression analysis in early and late erythroid progenitor cells in β-thalassaemia: an in vitro comparative study between normal and β-Thalassaemia erythroblasts

AU - Forster, Luke Benjamin

PY - 2016

Y1 - 2016

N2 - This study examines erythropoiesis in B-thalassaemia major in a laboratory setting. A gene expression study demonstrated dysregulation of 384 genes in B-thalassaemia erythroblasts cultured for 14 days. An expected role for apoptosis and the cell cycle was however not clear at this stage and as such functional studies were performed which demonstrated no difference in apoptosis and a decrease in the proportion of cells in M-phase. Downregulation of the NF-KB pathway was demonstrated and as such efforts were made to inhibit this pathway in erythroblasts in vitro. This led to the discovery that RNF-187 is regulated by this pathway.

AB - This study examines erythropoiesis in B-thalassaemia major in a laboratory setting. A gene expression study demonstrated dysregulation of 384 genes in B-thalassaemia erythroblasts cultured for 14 days. An expected role for apoptosis and the cell cycle was however not clear at this stage and as such functional studies were performed which demonstrated no difference in apoptosis and a decrease in the proportion of cells in M-phase. Downregulation of the NF-KB pathway was demonstrated and as such efforts were made to inhibit this pathway in erythroblasts in vitro. This led to the discovery that RNF-187 is regulated by this pathway.

KW - B-thalassaemia

KW - Microarray

KW - Quantitative real-time PCR

KW - Gene expression

KW - Erythropoiesis

KW - Apoptosis

KW - Cell cycle

KW - Proliferation

M3 - Doctoral Thesis

ER -

Differential gene expression analysis in early and late erythroid progenitor cells in β-thalassaemia: an in vitro comparative study between normal and β-Thalassaemia erythroblasts

Abstract

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