Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars

Tim Wherrett; Sergey Shabala; Igor Pottosin

doi:10.1016/j.febslet.2005.11.038

Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars

Tim Wherrett, Sergey Shabala, Igor Pottosin

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 μM vacuolar Al³⁺ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.

Original language	English
Pages (from-to)	6890-6894
Number of pages	5
Journal	FEBS Letters
Volume	579
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2005.11.038
Publication status	Published - 19 Dec 2005
Externally published	Yes

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10.1016/j.febslet.2005.11.038

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title = "Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars",

abstract = "Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 μM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.",

keywords = "Acid soil, Alt1, Calcium, Triticum aestivum, Vacuole",

author = "Tim Wherrett and Sergey Shabala and Igor Pottosin",

note = "Funding Information: This work was supported by ARC Discovery grant to S. Shabala and CONACyT 38181N grant to I. Pottosin.",

year = "2005",

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doi = "10.1016/j.febslet.2005.11.038",

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T1 - Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars

AU - Wherrett, Tim

AU - Shabala, Sergey

AU - Pottosin, Igor

N1 - Funding Information: This work was supported by ARC Discovery grant to S. Shabala and CONACyT 38181N grant to I. Pottosin.

PY - 2005/12/19

Y1 - 2005/12/19

N2 - Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 μM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.

AB - Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 μM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.

KW - Acid soil

KW - Alt1

KW - Calcium

KW - Triticum aestivum

KW - Vacuole

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DO - 10.1016/j.febslet.2005.11.038

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JO - FEBS Letters

JF - FEBS Letters

IS - 30

ER -

Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars

Abstract

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