Dietary Effects on the Development of Calliphora dubia and Chrysomya rufifacies (Diptera: Calliphoridae): Implications for Postmortem Interval

Ella K B Rogers, Daniel Franklin, Sasha C Voss

Research output: Contribution to journalArticle

Abstract

Forensic entomology relies on insect development data generated within a laboratory setting in the estimation of minimum postmortem interval (mPMI). The methodologies used to produce these data vary considerably within the field and there is no accepted standard approach to laboratory rearing of forensically relevant
species. A wide range of rearing media are used across published studies, including different species of animal and types of tissue (e.g., muscle and liver). Differing methodologies, particularly rearing diet, can introduce considerable variation into the baseline data upon which forensic estimates of the mPMI are calculated.
Consequently, research establishing a widely available, standard and/or optimal, rearing medium for blow fly
development for forensic application is desirable. This study examined dietary effects on the development of
two forensically relevant blow fly species: Calliphora dubia Macquart, 1855, and Chrysomya rufifacies Macquart
1842 (Diptera: Calliphoridae). Larvae of both species were reared on pork liver, pork mince, pork loin, beef liver,
beef mince, and guinea pig carcass under two constant temperature regimes (24 ± 1°C and 30 ± 1°C; 70 ± 10%
humidity; 12-h/12-h photoperiod) to assess the influence of temperature on developmental response to diet.
Fundamental developmental data pertaining to both species are reported. Developmental response to diet was
species-specific and influenced by temperature with indication that the optimal temperature for C. dubia development is below 30°C. Pork mince was the most appropriate dietary standard of the rearing media investigated
for the formulation of forensic development data for both species investigated.
Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalJournal of Medical Entomology
Volume58
Issue number1
Early online date29 Jun 2020
DOIs
Publication statusPublished - Jan 2021

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