Diel O2 Dynamics in Partially and Completely Submerged Deepwater Rice: Leaf Gas Films Enhance Internodal O2 Status, Influence Gene Expression and Accelerate Stem Elongation for 'Snorkelling' during Submergence

Yoshinao Mori, Yusuke Kurokawa, Masaya Koike, Al Imran Malik, Timothy David Colmer, Motoyuki Ashikari, Ole Pedersen, Keisuke Nagai

Research output: Contribution to journalArticle

Abstract

Deepwater rice has a remarkable shoot elongation response to partial submergence. Shoot elongation to maintain air-contact enables 'snorkelling' of O2 to submerged organs. Previous research has focused on partial submergence of deepwater rice. We tested the hypothesis that leaf gas films enhance internode O2 status and stem elongation of deepwater rice when completely submerged. Diel patterns of O2 partial pressure (pO2) were measured in internodes of deepwater rice when partially or completely submerged, and with or without gas films on leaves, for the completely submerged plants. We also took measurements for paddy rice. Deepwater rice elongated during complete submergence and the shoot tops emerged. Leaf gas films improved O2 entry during the night, preventing anoxia in stems, which is of importance for elongation of the submerged shoots. Expressions of O2 deprivation inducible genes were upregulated in completely submerged plants during the night, and more so when gas films were removed from the leaves. Diel O2 dynamics showed similar patterns in paddy and deepwater rice. We demonstrated that shoot tops in air enabled 'snorkelling' and increased O2 in internodes of both rice ecotypes; however, 'snorkelling' was achieved only by rapid shoot elongation by deepwater rice, but not by paddy rice.

Original languageEnglish
Pages (from-to)973-985
Number of pages13
JournalPlant & Cell Physiology
Volume60
Issue number5
DOIs
Publication statusPublished - 1 May 2019

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submergence
stem elongation
Gases
gases
Gene Expression
rice
gene expression
leaves
shoots
internodes
submerged aquatic plants
Air
Oryza
Ecotype
air
Partial Pressure
ecotypes
paddies
hypoxia
stems

Cite this

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title = "Diel O2 Dynamics in Partially and Completely Submerged Deepwater Rice: Leaf Gas Films Enhance Internodal O2 Status, Influence Gene Expression and Accelerate Stem Elongation for 'Snorkelling' during Submergence",
abstract = "Deepwater rice has a remarkable shoot elongation response to partial submergence. Shoot elongation to maintain air-contact enables 'snorkelling' of O2 to submerged organs. Previous research has focused on partial submergence of deepwater rice. We tested the hypothesis that leaf gas films enhance internode O2 status and stem elongation of deepwater rice when completely submerged. Diel patterns of O2 partial pressure (pO2) were measured in internodes of deepwater rice when partially or completely submerged, and with or without gas films on leaves, for the completely submerged plants. We also took measurements for paddy rice. Deepwater rice elongated during complete submergence and the shoot tops emerged. Leaf gas films improved O2 entry during the night, preventing anoxia in stems, which is of importance for elongation of the submerged shoots. Expressions of O2 deprivation inducible genes were upregulated in completely submerged plants during the night, and more so when gas films were removed from the leaves. Diel O2 dynamics showed similar patterns in paddy and deepwater rice. We demonstrated that shoot tops in air enabled 'snorkelling' and increased O2 in internodes of both rice ecotypes; however, 'snorkelling' was achieved only by rapid shoot elongation by deepwater rice, but not by paddy rice.",
keywords = "Oryza sativa, Air film, Anoxia, Flood tolerance, Internal aeration, Internode elongation, Paddy rice, Superhydrophobic leaves, Tissue oxygen partial pressure, Underwater photosynthesis",
author = "Yoshinao Mori and Yusuke Kurokawa and Masaya Koike and Malik, {Al Imran} and Colmer, {Timothy David} and Motoyuki Ashikari and Ole Pedersen and Keisuke Nagai",
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TY - JOUR

T1 - Diel O2 Dynamics in Partially and Completely Submerged Deepwater Rice

T2 - Leaf Gas Films Enhance Internodal O2 Status, Influence Gene Expression and Accelerate Stem Elongation for 'Snorkelling' during Submergence

AU - Mori, Yoshinao

AU - Kurokawa, Yusuke

AU - Koike, Masaya

AU - Malik, Al Imran

AU - Colmer, Timothy David

AU - Ashikari, Motoyuki

AU - Pedersen, Ole

AU - Nagai, Keisuke

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KW - Oryza sativa

KW - Air film

KW - Anoxia

KW - Flood tolerance

KW - Internal aeration

KW - Internode elongation

KW - Paddy rice

KW - Superhydrophobic leaves

KW - Tissue oxygen partial pressure

KW - Underwater photosynthesis

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SN - 0032-0781

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