Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 130-135 |
| Journal | Journal of Pediatric Gastroenterology and Nutrition |
| Volume | 47 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 |
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Diagnosis of Helicobacter pylori Infection in a High-prevalence Pediatric Population: A Comparison of 2 Fecal Antigen Testing Methods and Serology. / Cherian, Sarah; Burgner, David; Carson, Christine; Sanfilippo, Frank; Cook, Angus; Forbes, David.
In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 47, No. 2, 2008, p. 130-135.Research output: Contribution to journal › Article
TY - JOUR
T1 - Diagnosis of Helicobacter pylori Infection in a High-prevalence Pediatric Population: A Comparison of 2 Fecal Antigen Testing Methods and Serology
AU - Cherian, Sarah
AU - Burgner, David
AU - Carson, Christine
AU - Sanfilippo, Frank
AU - Cook, Angus
AU - Forbes, David
PY - 2008
Y1 - 2008
N2 - Objectives: Accurate methods for diagnosing active Helicobacter pylori infection in children have been limited to invasive or time-consuming techniques. Recently, fecal antigen testing has been used successfully for the diagnosis of H pylori infection in the pediatric population. We compared 2 monoclonal fecal antigen diagnostic methods in a population of children with a suspected high prevalence of H pylori infection. We also assessed the diagnostic performance of H pylori immunoglobulin G scrology.Materials and Methods: In a cross-sectional study of African refugee children (< 16 years) we compared an immunochromatographic technique (ICT) and serology with a monoclonal fecal antigen enzyme immunoassay (MFAT) method for the detection of active H pylori infection. Following the manufacturer's instructions, an optical density of >= 0.190 was used as a cutoff for MFAT. Sensitivity, specificity, and positive and negative predictive values were calculated.Results: Of the 193 eligible children enrolled, active H pylori infection was detected in 149 of 182 (81.9%) in whom MFAT was performed. The prevalence of active infection increased with age; children with active infection were significantly older, and there were no sex differences. ICT and serology underperformed in comparison with MFAT (ICT sensitivity 74.6%, specificity 63.6%, positive predictive value 89.8%, negative predictive value 36.8%; and serology sensitivity 57.9%, specificity 77.4%, positive predictive value 92.0%, negative predictive value 29.9%).Conclusions: Monoclonal enzyme immunoassay fecal antigen testing is a practical and feasible alternative to traditional invasive diagnostic methods in high-prevalence pediatric populations. Neither immunochromatography nor serology is useful for the diagnosis of active H pylori infection in these children.
AB - Objectives: Accurate methods for diagnosing active Helicobacter pylori infection in children have been limited to invasive or time-consuming techniques. Recently, fecal antigen testing has been used successfully for the diagnosis of H pylori infection in the pediatric population. We compared 2 monoclonal fecal antigen diagnostic methods in a population of children with a suspected high prevalence of H pylori infection. We also assessed the diagnostic performance of H pylori immunoglobulin G scrology.Materials and Methods: In a cross-sectional study of African refugee children (< 16 years) we compared an immunochromatographic technique (ICT) and serology with a monoclonal fecal antigen enzyme immunoassay (MFAT) method for the detection of active H pylori infection. Following the manufacturer's instructions, an optical density of >= 0.190 was used as a cutoff for MFAT. Sensitivity, specificity, and positive and negative predictive values were calculated.Results: Of the 193 eligible children enrolled, active H pylori infection was detected in 149 of 182 (81.9%) in whom MFAT was performed. The prevalence of active infection increased with age; children with active infection were significantly older, and there were no sex differences. ICT and serology underperformed in comparison with MFAT (ICT sensitivity 74.6%, specificity 63.6%, positive predictive value 89.8%, negative predictive value 36.8%; and serology sensitivity 57.9%, specificity 77.4%, positive predictive value 92.0%, negative predictive value 29.9%).Conclusions: Monoclonal enzyme immunoassay fecal antigen testing is a practical and feasible alternative to traditional invasive diagnostic methods in high-prevalence pediatric populations. Neither immunochromatography nor serology is useful for the diagnosis of active H pylori infection in these children.
U2 - 10.1097/MPG.0b013e31815bc5b3
DO - 10.1097/MPG.0b013e31815bc5b3
M3 - Article
VL - 47
SP - 130
EP - 135
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - 2
ER -