Developmental analysis of transformation in legumes

Hoai An Nguyen

Research output: ThesisDoctoral Thesis

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Abstract

Efforts to improve crop legume germplasm by use of genetic technologies are hampered globally by the lack of an efficient genetic transformation method for most legume species. It was hypothesised that a fundamental investigation of shoot development would provide vital information to improve the transformation outcomes. This research investigated alternative selection methodologies, and used reporter genes to visualise shoot development to overcome the limitations of low frequency and chimerism of transgenic shoots. The results demonstrated that development of a high yielding transformation methodology for pulse legume crops is achievable and applicable to other legume species that are recalcitrant to transformation.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Barker, Susan Jane, Supervisor
  • Erskine, William, Supervisor
Award date9 Jan 2018
DOIs
Publication statusUnpublished - 2017

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