Filamentous fungi are a major source of secondary metabolites with biotechnological applications. The aim of this thesis is to develop synthetic biology tools to accelerate the discovery of fungal compounds. First, a system of artificial transcription factors based on CRISPR/Cas (CRISPRa) was developed to activate the expression of silent biosynthetic pathways. Second, a system for targeted chromosomal integration of large biosynthetic gene clusters using site-specific recombinases was developed. Additionally, the biosynthesis of the polyketide burnettiene A was investigated by heterologous expression in Aspergillus nidulans. The tools developed in this thesis represent a valuable expansion to the filamentous fungi synthetic biology toolbox.
|Qualification||Doctor of Philosophy|
|Award date||20 May 2021|
|Publication status||Unpublished - 2021|
Take-down noticeEmbargoed from 30/05/2021 to 30/08/2021
Made publicly available on 30/08/2021