Development of synthetic biology tools for accelerating fungal natural product discovery

Research output: ThesisDoctoral Thesis

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Abstract

Filamentous fungi are a major source of secondary metabolites with biotechnological applications. The aim of this thesis is to develop synthetic biology tools to accelerate the discovery of fungal compounds. First, a system of artificial transcription factors based on CRISPR/Cas (CRISPRa) was developed to activate the expression of silent biosynthetic pathways. Second, a system for targeted chromosomal integration of large biosynthetic gene clusters using site-specific recombinases was developed. Additionally, the biosynthesis of the polyketide burnettiene A was investigated by heterologous expression in Aspergillus nidulans. The tools developed in this thesis represent a valuable expansion to the filamentous fungi synthetic biology toolbox.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Chooi, Heng, Supervisor
  • Mylne, Josh, Supervisor
  • Waters, Mark, Supervisor
Award date20 May 2021
DOIs
Publication statusUnpublished - 2021

Take-down notice

Embargoed from 30/05/2021 to 30/08/2021
Made publicly available on 30/08/2021

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