Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe Toxica in narrow-leafed lupin (Lupinus angustifolius L.)

H. Yang; Manisha Shankar; B.J. Buirchell; M.W. Sweetingham; C. Caminero; P.M.C. Smith

doi:10.1007/s00122-002-0925-1

Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe Toxica in narrow-leafed lupin (Lupinus angustifolius L.)

H. Yang, Manisha Shankar, B.J. Buirchell, M.W. Sweetingham, C. Caminero, P.M.C. Smith

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Phomopsis stem blight (PSB) caused by Diaporthe toxica is a major disease in narrow-leafed lupin (Lupinus angustifolius L.). The F-2 progeny and the parental plants from a cross between a breeding line 75A:258 (containing a single dominant resistance gene Phr1 against the disease) and a commercial cultivar Unicrop (susceptible to the disease) were used for development of molecular markers linked to the disease resistance gene. Two pairs of co-dominant DNA polymorphisms were detected using the microsatellite-anchored fragment length polymorphism (MFLP) technique. Both pairs of polymorphisms were isolated from the MFLP gels, re-amplified by PCR, sequenced, and converted into co-dominant, sequence-specific and PCR-based markers. Linkage analysis by MAPMAKER suggested that one marker (Ph258M2) was 5.7 centiMorgans (cM) from Phr1, and the other marker (Ph258M1) was 2.1 cM from Ph258M2 but further away from Phr1. These markers are suitable for marker-assisted selection (MAS) in lupin breeding.

Original language	English
Pages (from-to)	265-270
Journal	Theoretical and Applied Genetics
Volume	105
DOIs	https://doi.org/10.1007/s00122-002-0925-1
Publication status	Published - 2002

Access to Document

10.1007/s00122-002-0925-1

Cite this

@article{ae8e1077947948279a21e305241ee7ed,

title = "Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe Toxica in narrow-leafed lupin (Lupinus angustifolius L.)",

abstract = "Phomopsis stem blight (PSB) caused by Diaporthe toxica is a major disease in narrow-leafed lupin (Lupinus angustifolius L.). The F-2 progeny and the parental plants from a cross between a breeding line 75A:258 (containing a single dominant resistance gene Phr1 against the disease) and a commercial cultivar Unicrop (susceptible to the disease) were used for development of molecular markers linked to the disease resistance gene. Two pairs of co-dominant DNA polymorphisms were detected using the microsatellite-anchored fragment length polymorphism (MFLP) technique. Both pairs of polymorphisms were isolated from the MFLP gels, re-amplified by PCR, sequenced, and converted into co-dominant, sequence-specific and PCR-based markers. Linkage analysis by MAPMAKER suggested that one marker (Ph258M2) was 5.7 centiMorgans (cM) from Phr1, and the other marker (Ph258M1) was 2.1 cM from Ph258M2 but further away from Phr1. These markers are suitable for marker-assisted selection (MAS) in lupin breeding.",

author = "H. Yang and Manisha Shankar and B.J. Buirchell and M.W. Sweetingham and C. Caminero and P.M.C. Smith",

year = "2002",

doi = "10.1007/s00122-002-0925-1",

language = "English",

volume = "105",

pages = "265--270",

journal = "Theoretical and Applied Genetics",

issn = "0040-5752",

publisher = "Springer",

}

TY - JOUR

T1 - Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe Toxica in narrow-leafed lupin (Lupinus angustifolius L.)

AU - Yang, H.

AU - Shankar, Manisha

AU - Buirchell, B.J.

AU - Sweetingham, M.W.

AU - Caminero, C.

AU - Smith, P.M.C.

PY - 2002

Y1 - 2002

N2 - Phomopsis stem blight (PSB) caused by Diaporthe toxica is a major disease in narrow-leafed lupin (Lupinus angustifolius L.). The F-2 progeny and the parental plants from a cross between a breeding line 75A:258 (containing a single dominant resistance gene Phr1 against the disease) and a commercial cultivar Unicrop (susceptible to the disease) were used for development of molecular markers linked to the disease resistance gene. Two pairs of co-dominant DNA polymorphisms were detected using the microsatellite-anchored fragment length polymorphism (MFLP) technique. Both pairs of polymorphisms were isolated from the MFLP gels, re-amplified by PCR, sequenced, and converted into co-dominant, sequence-specific and PCR-based markers. Linkage analysis by MAPMAKER suggested that one marker (Ph258M2) was 5.7 centiMorgans (cM) from Phr1, and the other marker (Ph258M1) was 2.1 cM from Ph258M2 but further away from Phr1. These markers are suitable for marker-assisted selection (MAS) in lupin breeding.

AB - Phomopsis stem blight (PSB) caused by Diaporthe toxica is a major disease in narrow-leafed lupin (Lupinus angustifolius L.). The F-2 progeny and the parental plants from a cross between a breeding line 75A:258 (containing a single dominant resistance gene Phr1 against the disease) and a commercial cultivar Unicrop (susceptible to the disease) were used for development of molecular markers linked to the disease resistance gene. Two pairs of co-dominant DNA polymorphisms were detected using the microsatellite-anchored fragment length polymorphism (MFLP) technique. Both pairs of polymorphisms were isolated from the MFLP gels, re-amplified by PCR, sequenced, and converted into co-dominant, sequence-specific and PCR-based markers. Linkage analysis by MAPMAKER suggested that one marker (Ph258M2) was 5.7 centiMorgans (cM) from Phr1, and the other marker (Ph258M1) was 2.1 cM from Ph258M2 but further away from Phr1. These markers are suitable for marker-assisted selection (MAS) in lupin breeding.

U2 - 10.1007/s00122-002-0925-1

DO - 10.1007/s00122-002-0925-1

M3 - Article

SN - 0040-5752

VL - 105

SP - 265

EP - 270

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

ER -

Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe Toxica in narrow-leafed lupin (Lupinus angustifolius L.)

Abstract

Access to Document

Fingerprint

Cite this