Development of microsatellites from the rare ironstone endemic, Tetratheca paynterae ssp. paynterae and cross-species amplification

P.A. Butcher, Siegfried Krauss

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    Abstract

    Nineteen microsatellite markers were developed from Tetratheca paynterae ssp. paynterae, a rare and endangered, leafless, perennial shrub. Twelve loci were polymorphic in T. paynterae ssp. paynterae with two to 14 alleles per locus and mean expected heterozygosity of 0.62. Primer pairs were tested on four other Tetratheca species from ironstone ranges in southern Western Australia. Ten loci were polymorphic in T. paynterae ssp. cremnobata and T. aphylla ssp. aphylla, three in T. harperi and four in T. erubescens. The level of polymorphism was adequate for studies of genetic structure and mating systems in three of the five taxa.
    Original languageEnglish
    Pages (from-to)386-389
    JournalMolecular Ecology Resources
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - 2009

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