Development of KASP markers and identification of candidate genes for pre-harvest sprouting, drought and herbicide tolerance for molecular assisted breeding in wheat

Guannan Liu

doi:10.26182/g7g6-zt10

Development of KASP markers and identification of candidate genes for pre-harvest sprouting, drought and herbicide tolerance for molecular assisted breeding in wheat

Guannan Liu

UWA School of Agriculture and Environment

Research output: Thesis › Doctoral Thesis

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Abstract

Common wheat is an important crop for global food security and it faces stresses like pre-harvest sprouting, drought, and weeds. Developing new, stress-tolerant wheat varieties is essential and molecular marker-assisted breeding is needed. However, the complex wheat genome and quantitative traits of these stresses pose challenges. Two innovative approaches (SQV, SRV) were developed for efficient and cost-effective KASP markers. These techniques involve genotyping (S)selected NILs or RILs (Q or R) for specific traits and validation with commercial varieties (V). This approach successfully identified practical KASP markers and candidate genes for stress tolerance wheat breeding and genetic research.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Liu, Hui, Supervisor Yan, Guijun, Supervisor Liu, Dongcheng, Supervisor, External person Mullan, Daniel, Supervisor Zhang, Aimin, Supervisor, External person
Thesis sponsors	Australian Government Research Training Program
Award date	10 Jun 2024
DOIs	https://doi.org/10.26182/g7g6-zt10
Publication status	Unpublished - 2024

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THESIS - DOCTOR OF PHILOSOPHY - LIU Guannan - 2024_
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Cite this

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title = "Development of KASP markers and identification of candidate genes for pre-harvest sprouting, drought and herbicide tolerance for molecular assisted breeding in wheat",

abstract = "Common wheat is an important crop for global food security and it faces stresses like pre-harvest sprouting, drought, and weeds. Developing new, stress-tolerant wheat varieties is essential and molecular marker-assisted breeding is needed. However, the complex wheat genome and quantitative traits of these stresses pose challenges. Two innovative approaches (SQV, SRV) were developed for efficient and cost-effective KASP markers. These techniques involve genotyping (S)selected NILs or RILs (Q or R) for specific traits and validation with commercial varieties (V). This approach successfully identified practical KASP markers and candidate genes for stress tolerance wheat breeding and genetic research.",

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AU - Liu, Guannan

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Y1 - 2024

N2 - Common wheat is an important crop for global food security and it faces stresses like pre-harvest sprouting, drought, and weeds. Developing new, stress-tolerant wheat varieties is essential and molecular marker-assisted breeding is needed. However, the complex wheat genome and quantitative traits of these stresses pose challenges. Two innovative approaches (SQV, SRV) were developed for efficient and cost-effective KASP markers. These techniques involve genotyping (S)selected NILs or RILs (Q or R) for specific traits and validation with commercial varieties (V). This approach successfully identified practical KASP markers and candidate genes for stress tolerance wheat breeding and genetic research.

AB - Common wheat is an important crop for global food security and it faces stresses like pre-harvest sprouting, drought, and weeds. Developing new, stress-tolerant wheat varieties is essential and molecular marker-assisted breeding is needed. However, the complex wheat genome and quantitative traits of these stresses pose challenges. Two innovative approaches (SQV, SRV) were developed for efficient and cost-effective KASP markers. These techniques involve genotyping (S)selected NILs or RILs (Q or R) for specific traits and validation with commercial varieties (V). This approach successfully identified practical KASP markers and candidate genes for stress tolerance wheat breeding and genetic research.

KW - KASP marker

KW - Functional genes

KW - 90K SNP assay

KW - Near-isogenic lines

KW - Wheat

KW - Recombinant inbred lines

KW - DArT sequencing

U2 - 10.26182/g7g6-zt10

DO - 10.26182/g7g6-zt10

M3 - Doctoral Thesis

ER -

Development of KASP markers and identification of candidate genes for pre-harvest sprouting, drought and herbicide tolerance for molecular assisted breeding in wheat

Abstract

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