Development of Immuno-flowFISH for detection of del(17p) in Chronic Lymphocytic Leukaemia

Jason Stanley

doi:10.26182/dwf7-rk91

Development of Immuno-flowFISH for detection of del(17p) in Chronic Lymphocytic Leukaemia

Jason Stanley

Research output: Thesis › Doctoral Thesis

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Abstract

Deletion of the short arm of chromosome 17 (del(17p)) in chronic lymphocytic leukaemia occurs in 5-8% of patients and is associated with poor response to chemotherapy. Fluorescent in situ hybridisation (FISH) is performed prior to treatment to detect this abnormality to ensure optimal therapy is given. However, FISH lacks sensitivity (5%) meaning this critical lesion may be undetected. I developed an approach that combines immunophenotypic identification of the leukaemic cells with FISH, and imaging flow cytometric analysis. This technique, with a limit of detection of 10-5 offers potential for detection of rare del(17p) clones at diagnosis and throughout the disease.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Fuller, Kathy, Supervisor Erber, Wendy, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	9 Jun 2023
DOIs	https://doi.org/10.26182/dwf7-rk91
Publication status	Unpublished - 2023

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10.26182/dwf7-rk91

THESIS - DOCTOR OF PHILOSOPHY - STANLEY Jason - 2023
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1 Finished

Precision molecular diagnostics of single cells by imaging flow cytometry
Fuller, K. (Investigator 01), Erber, W. (Investigator 02), Hui, H. (Investigator 03) & Dickinson, J. (Investigator 04)
NHMRC National Health and Medical Research Council
1/01/21 → 31/12/24
Project: Research

1 Review article

Analysis of human chromosomes by imaging flow cytometry
Stanley, J., Hui, H., Erber, W., Clynick, B. & Fuller, K., Sept 2021, In: Cytometry Part B - Clinical Cytometry. 100, 5, p. 541-553 13 p.
Research output: Contribution to journal › Review article › peer-review
5 Citations (Web of Science)

Cite this

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title = "Development of Immuno-flowFISH for detection of del(17p) in Chronic Lymphocytic Leukaemia",

abstract = "Deletion of the short arm of chromosome 17 (del(17p)) in chronic lymphocytic leukaemia occurs in 5-8% of patients and is associated with poor response to chemotherapy. Fluorescent in situ hybridisation (FISH) is performed prior to treatment to detect this abnormality to ensure optimal therapy is given. However, FISH lacks sensitivity (5%) meaning this critical lesion may be undetected. I developed an approach that combines immunophenotypic identification of the leukaemic cells with FISH, and imaging flow cytometric analysis. This technique, with a limit of detection of 10-5 offers potential for detection of rare del(17p) clones at diagnosis and throughout the disease.",

keywords = "Chronic Lymphocytic Leukaemia, Imaging Flow Cytometry, Cytogenetics, del(17p), Fluorescence in situ hybridisation, Haematological Malignancies",

author = "Jason Stanley",

year = "2023",

doi = "10.26182/dwf7-rk91",

language = "English",

school = "The University of Western Australia",

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T1 - Development of Immuno-flowFISH for detection of del(17p) in Chronic Lymphocytic Leukaemia

AU - Stanley, Jason

PY - 2023

Y1 - 2023

N2 - Deletion of the short arm of chromosome 17 (del(17p)) in chronic lymphocytic leukaemia occurs in 5-8% of patients and is associated with poor response to chemotherapy. Fluorescent in situ hybridisation (FISH) is performed prior to treatment to detect this abnormality to ensure optimal therapy is given. However, FISH lacks sensitivity (5%) meaning this critical lesion may be undetected. I developed an approach that combines immunophenotypic identification of the leukaemic cells with FISH, and imaging flow cytometric analysis. This technique, with a limit of detection of 10-5 offers potential for detection of rare del(17p) clones at diagnosis and throughout the disease.

AB - Deletion of the short arm of chromosome 17 (del(17p)) in chronic lymphocytic leukaemia occurs in 5-8% of patients and is associated with poor response to chemotherapy. Fluorescent in situ hybridisation (FISH) is performed prior to treatment to detect this abnormality to ensure optimal therapy is given. However, FISH lacks sensitivity (5%) meaning this critical lesion may be undetected. I developed an approach that combines immunophenotypic identification of the leukaemic cells with FISH, and imaging flow cytometric analysis. This technique, with a limit of detection of 10-5 offers potential for detection of rare del(17p) clones at diagnosis and throughout the disease.

KW - Chronic Lymphocytic Leukaemia

KW - Imaging Flow Cytometry

KW - Cytogenetics

KW - del(17p)

KW - Fluorescence in situ hybridisation

KW - Haematological Malignancies

U2 - 10.26182/dwf7-rk91

DO - 10.26182/dwf7-rk91

M3 - Doctoral Thesis

ER -

Development of Immuno-flowFISH for detection of del(17p) in Chronic Lymphocytic Leukaemia

Abstract

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Projects

Precision molecular diagnostics of single cells by imaging flow cytometry

Research output

Analysis of human chromosomes by imaging flow cytometry

Cite this