Development of Immuno-flowFISH for detection of del(17p) in Chronic Lymphocytic Leukaemia

Jason Stanley

Research output: ThesisDoctoral Thesis

185 Downloads (Pure)

Abstract

Deletion of the short arm of chromosome 17 (del(17p)) in chronic lymphocytic leukaemia occurs in 5-8% of patients and is associated with poor response to chemotherapy. Fluorescent in situ hybridisation (FISH) is performed prior to treatment to detect this abnormality to ensure optimal therapy is given. However, FISH lacks sensitivity (5%) meaning this critical lesion may be undetected. I developed an approach that combines immunophenotypic identification of the leukaemic cells with FISH, and imaging flow cytometric analysis. This technique, with a limit of detection of 10-5 offers potential for detection of rare del(17p) clones at diagnosis and throughout the disease.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Fuller, Kathy, Supervisor
  • Erber, Wendy, Supervisor
Thesis sponsors
Award date9 Jun 2023
DOIs
Publication statusUnpublished - 2023

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