Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration

A. Kaczmarczyk; B. Funnekotter; Shane Turner; Eric Bunn; G. Bryant; T.E. Hunt; R.L. Mancera

Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration

A. Kaczmarczyk, B. Funnekotter, Shane Turner, Eric Bunn, G. Bryant, T.E. Hunt, R.L. Mancera

Research output: Contribution to journal › Article

Abstract

We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. © CryoLetters.

Original language	English
Pages (from-to)	508-519
Journal	Cryo-Letters
Volume	34
Issue number	5
Publication status	Published - 2013

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@article{7c39bde375d4453a8be82066dff820ec,

title = "Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration",

abstract = "We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. {\textcopyright} CryoLetters.",

author = "A. Kaczmarczyk and B. Funnekotter and Shane Turner and Eric Bunn and G. Bryant and T.E. Hunt and R.L. Mancera",

year = "2013",

language = "English",

volume = "34",

pages = "508--519",

journal = "Cryoletters",

issn = "0143-2044",

publisher = "Cryo-Letters",

number = "5",

}

TY - JOUR

T1 - Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration

AU - Kaczmarczyk, A.

AU - Funnekotter, B.

AU - Turner, Shane

AU - Bunn, Eric

AU - Bryant, G.

AU - Hunt, T.E.

AU - Mancera, R.L.

PY - 2013

Y1 - 2013

N2 - We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. © CryoLetters.

AB - We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. © CryoLetters.

M3 - Article

VL - 34

SP - 508

EP - 519

JO - Cryoletters

JF - Cryoletters

SN - 0143-2044

IS - 5

ER -