Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 508-519 |
| Journal | Cryo-Letters |
| Volume | 34 |
| Issue number | 5 |
| Publication status | Published - 2013 |
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Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration. / Kaczmarczyk, A.; Funnekotter, B.; Turner, Shane; Bunn, Eric; Bryant, G.; Hunt, T.E.; Mancera, R.L.
In: Cryo-Letters, Vol. 34, No. 5, 2013, p. 508-519.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration
AU - Kaczmarczyk, A.
AU - Funnekotter, B.
AU - Turner, Shane
AU - Bunn, Eric
AU - Bryant, G.
AU - Hunt, T.E.
AU - Mancera, R.L.
PY - 2013
Y1 - 2013
N2 - We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. © CryoLetters.
AB - We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. © CryoLetters.
M3 - Article
VL - 34
SP - 508
EP - 519
JO - Cryoletters
JF - Cryoletters
SN - 0143-2044
IS - 5
ER -