Development of cryopreservation for Loxocarya cinerea - An endemic Australian plant species important for post-mining restoration

A. Kaczmarczyk, B. Funnekotter, Shane Turner, Eric Bunn, G. Bryant, T.E. Hunt, R.L. Mancera

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    Abstract

    We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. © CryoLetters.
    Original languageEnglish
    Pages (from-to)508-519
    JournalCryo-Letters
    Volume34
    Issue number5
    Publication statusPublished - 2013

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