TY - JOUR
T1 - Development of a simple and effective silver staining protocol for detection of DNA fragments
AU - Liu, Wenjie
AU - Li, Ronghua
AU - Ayalew, Habtamu
AU - Xia, Yanshi
AU - Bai, Guihua
AU - Yan, Guijun
AU - Siddique, Kadambot H M
AU - Guo, Peiguo
PY - 2017/4
Y1 - 2017/4
N2 - Silver staining is one of the widely used methods for DNA fragment detection in biological research. Silver staining protocols have been steadily optimized to improve detection efficiency. This research reports a continuous effort to simplify the existing silver staining protocols, lower experiment cost, and improve DNA detection sensitivity and image clarity. The new method only requires three reagents (silver nitrate, sodium hydroxide, and formaldehyde) and 6-7 min with high detection sensitivity to visualize as low as 14.6 pg (3.3 pg/mm2) of DNA in a non-denaturing polyacrylamide gel. In comparison to previous reported protocols, the new one has the highest resolution, is the easiest to operate, takes the shortest time, and uses the fewest chemical reagents. Therefore, the new method can be used for quick generation of high quality molecular marker data in genetic analysis.
AB - Silver staining is one of the widely used methods for DNA fragment detection in biological research. Silver staining protocols have been steadily optimized to improve detection efficiency. This research reports a continuous effort to simplify the existing silver staining protocols, lower experiment cost, and improve DNA detection sensitivity and image clarity. The new method only requires three reagents (silver nitrate, sodium hydroxide, and formaldehyde) and 6-7 min with high detection sensitivity to visualize as low as 14.6 pg (3.3 pg/mm2) of DNA in a non-denaturing polyacrylamide gel. In comparison to previous reported protocols, the new one has the highest resolution, is the easiest to operate, takes the shortest time, and uses the fewest chemical reagents. Therefore, the new method can be used for quick generation of high quality molecular marker data in genetic analysis.
KW - DNA
KW - Non-denaturing polyacrylamide gel
KW - Silver staining
UR - http://www.scopus.com/inward/record.url?scp=85013392336&partnerID=8YFLogxK
U2 - 10.1002/elps.201700009
DO - 10.1002/elps.201700009
M3 - Article
C2 - 28145034
AN - SCOPUS:85013392336
SN - 0173-0835
VL - 38
SP - 1175
EP - 1178
JO - Electrophoresis
JF - Electrophoresis
IS - 8
ER -