Development of a PCR marker tightly linked to mollis, the gene that controls seed dormancy in Lupinus angustifolius L.

J.G. Boersma, B.J. Buirchell, Krishnapillai Sivasithamparam, H. Yang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Wild plants of Lupinus angustifolius avoid extinction in a drought year by production of seeds with coats that are impermeable to water, preventing germination of a large percentage of the seed in any given year. Domesticated cultivars of this species carry the recessive gene mollis, making the seed coat permeable to water and, in turn promoting good crop establishment in the year of sowing. A dominant microsatellite-anchored fragment length polymorphism candidate marker was identified as being tightly linked to mollis in a population of recombinant inbred lines derived from domesticated and wild-type parents. The candidate marker was excised from the gel, amplified by PCR, sequenced and extended beyond the SSR end of the original MseI-SSR fragment. Two single nucleotide polymorphisms were found within this extended sequence. Specific primers were designed to create a marker 209 bp long. PCR products of these primers run on a single strand conformation polymorphism gel resolved in a co-dominant fashion. This marker will be used in marker-assisted selection for mollis when introgressing wild material into lupin breeding programmes.
Original languageEnglish
Pages (from-to)612-616
JournalPlant Breeding
Volume126
Issue number6
DOIs
Publication statusPublished - 2007

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Plant Dormancy
Lupinus
Lupinus angustifolius
seed dormancy
Seeds
gels
Polymerase Chain Reaction
single-stranded conformational polymorphism
recessive genes
plant establishment
wild plants
Gels
marker-assisted selection
inbred lines
single nucleotide polymorphism
Genes
Recessive Genes
extinction
genes
sowing

Cite this

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title = "Development of a PCR marker tightly linked to mollis, the gene that controls seed dormancy in Lupinus angustifolius L.",
abstract = "Wild plants of Lupinus angustifolius avoid extinction in a drought year by production of seeds with coats that are impermeable to water, preventing germination of a large percentage of the seed in any given year. Domesticated cultivars of this species carry the recessive gene mollis, making the seed coat permeable to water and, in turn promoting good crop establishment in the year of sowing. A dominant microsatellite-anchored fragment length polymorphism candidate marker was identified as being tightly linked to mollis in a population of recombinant inbred lines derived from domesticated and wild-type parents. The candidate marker was excised from the gel, amplified by PCR, sequenced and extended beyond the SSR end of the original MseI-SSR fragment. Two single nucleotide polymorphisms were found within this extended sequence. Specific primers were designed to create a marker 209 bp long. PCR products of these primers run on a single strand conformation polymorphism gel resolved in a co-dominant fashion. This marker will be used in marker-assisted selection for mollis when introgressing wild material into lupin breeding programmes.",
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journal = "Plant Breeding",
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Development of a PCR marker tightly linked to mollis, the gene that controls seed dormancy in Lupinus angustifolius L. / Boersma, J.G.; Buirchell, B.J.; Sivasithamparam, Krishnapillai; Yang, H.

In: Plant Breeding, Vol. 126, No. 6, 2007, p. 612-616.

Research output: Contribution to journalArticle

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AU - Yang, H.

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