TY - JOUR
T1 - Development and Validation of a Quantitative Analysis of Water-Soluble Vitamins Using High-Performance Thin-Layer Chromatography and Its Application to the Analysis of Nutraceuticals
AU - Sikdar, K. M. Yasif Kayes
AU - Islam, Md Khairul
AU - Sostaric, Tomislav
AU - Lim, Lee Yong
AU - Locher, Cornelia
PY - 2024/7/4
Y1 - 2024/7/4
N2 - In this study, a high-performance thin-layer chromatography (HPTLC)-based method was developed for the quantification of five water-soluble vitamins, B2, B3, B6, B12, and C, using ethanol– water (70:30, v/v) as the mobile phase. The method was validated according to the International Conference on Harmonisation (ICH) guidelines and the limits of detection/limits of quantification were determined as 5.27/15.97 ng (Vitamin B2), 13.03/39.47 ng (Vitamin B3), 59.61/180.64 ng (Vitamin B6), 14.17/42.93 ng (Vitamin B12), and 119.27/361.42 ng (Vitamin C) per band, respectively. The usefulness of the validated method for quality control was demonstrated with the successful quantitative analysis of the five vitamins in a range of nutraceuticals.
AB - In this study, a high-performance thin-layer chromatography (HPTLC)-based method was developed for the quantification of five water-soluble vitamins, B2, B3, B6, B12, and C, using ethanol– water (70:30, v/v) as the mobile phase. The method was validated according to the International Conference on Harmonisation (ICH) guidelines and the limits of detection/limits of quantification were determined as 5.27/15.97 ng (Vitamin B2), 13.03/39.47 ng (Vitamin B3), 59.61/180.64 ng (Vitamin B6), 14.17/42.93 ng (Vitamin B12), and 119.27/361.42 ng (Vitamin C) per band, respectively. The usefulness of the validated method for quality control was demonstrated with the successful quantitative analysis of the five vitamins in a range of nutraceuticals.
U2 - 10.3390/separations11070207
DO - 10.3390/separations11070207
M3 - Article
SN - 2227-9075
VL - 11
JO - Separations
JF - Separations
IS - 7
M1 - 207
ER -