Development and evaluation of curcumin-loaded Pluronic F127 nanoformulation

Suhaili Shamsi

    Research output: ThesisDoctoral Thesis

    1449 Downloads (Pure)

    Abstract

    [Truncated] Curcumin (CUR) is poorly water-soluble, which when combined with poor permeability and rapid metabolism, renders an extremely low oral bioavailability. The thesis sets out to support the hypothesis that a simple, safe and cost efficient CUR nanoformulation prepared with Pluronic F127 as an excipient would be stable on storage and in a myriad of biological media to retain the biological activities of CUR, and to improve the bioavailability of CUR.

    NanoCUR with size of 23.43 ± 0.18 nm and ability to encapsulate CUR at 95.15 ± 1.97% was successfully prepared by a thin dry film hydration (TDF) technique, and the dispersion was stable to lyophilisation followed by reconstitution in a range of aqueous media. The lyophilized product was stable to storage at room temperature and 4 °C for 12 months.

    Micelle carriers must be able to resist a rapid and premature dissociation upon dilution and exposure to the harsh and changing condition of the gastrointestinal tract (GIT). CUR-loaded Pluronic F127 nanoformulation (NanoCUR) was stable in the changing environmental pH of the GIT, with preserved size, morphology and CUR content in simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 6.8) after 72 h.

    The stability and characterization of nanoformulations play a significant role in determining the efficacy and performance in biological studies. However, there is no precedent work examining the long term stability and characterization of a CUR formulation at different concentrations or in various biological media. When dispersed in Eagle’s Minimum Essential Medium (EMEM) containing 10% Fetal Bovine Serum (FBS), NanoCUR showed a significantly smaller particle size than in water at concentrations of 2 to 120 μM. Dynamic light scattering (DLS) measurement suggested that both FBS, as well as the NanoCUR particles contribute to the size registered by DLS. The stability of NanoCUR was preserved with no apparent particle aggregates observed in EMEM supplemented with 10% FBS for 72 h.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Publication statusUnpublished - 2015

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