Development and characterization of near-isogenic lines revealing candidate genes for a major 7AL QTL responsible for heat tolerance in wheat

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Abstract

Wheat is one of the most important food crops in the world, but as a cool season crop it is more prone 10 to heat stress which severely affects production and grain quality of the crop. Heat tolerance in wheat 11 is a quantitative trait and genes underlying reported quantitative trait loci (QTLs) have rarely been 12 identified. Near-isogenic lines (NILs) with a common genetic background but differing at a particular 13 locus could turn quantitative traits into a Mendelian factor, therefore are suitable materials for 14 identification of candidate genes for targeted locus/loci. In this study, we developed and characterized 15 NILs from two populations Cascades × Tevere and Cascades × W156 targeting a major 7AL QTL 16 responsible for heat tolerance. Molecular marker screening and phenotyping for SPAD chlorophyll 17 content (SCC) and grain yield related traits confirmed four pairs of wheat NILs with significant contrast 18 for heat-stress responses between the isolines. Genotyping the NILs by 90K Infinium iSelect SNP array 19 revealed five single nucleotide polymorphism (SNP) markers within the QTL interval that were 20 distinguishable between the isolines. Seven candidate genes linked to the SNPs were identified as 21 related to heat tolerance. These genes were involved in important processes and pathways in response 22 to heat stress. The confirmed multiple pairs of NILs and identified candidate genes in this study are 23 valuable resources and information for further fine-mapping to clone the major genes for heat 24 tolerance.
Original languageEnglish
Article number1316
JournalFrontiers in Plant Science
Volume11
DOIs
Publication statusPublished - 28 Aug 2020

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