Development and characterisation of near-isogenic lines for metribuzin resistance in wheat (Triticumaestivum L.)

Rudra Bhattarai

doi:10.26182/4vp0-6d15

Development and characterisation of near-isogenic lines for metribuzin resistance in wheat (Triticumaestivum L.)

Rudra Bhattarai

Research output: Thesis › Doctoral Thesis

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Abstract

Metribuzin resistance in wheat is crucial for successful weed control. Near-isogenic lines (NILs) targeting a major metribuzin resistance locus explaining 69% of the phenotypic variance were developed and studied by mRNA sequencing and proteomic analyses, revealing nitrate excretion transporter, aspartyl protease, glycine-rich protein, WD repeats, AB hydrolase_1 and PsbP as major candidate genes/proteins associated with metribuzin resistance. A number of molecular markers associated with the candidate genes responsible for metribuzin resistance were identified, which can be validated and applied to marker-assisted selection (MAS) in wheat breeding while identified genes/proteins can help to understand genetic mechanism of metribuzin resistance in wheat.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Liu, Hui, Supervisor Siddique, Kadambot, Supervisor Yan, Guijun, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	30 Aug 2023
DOIs	https://doi.org/10.26182/4vp0-6d15
Publication status	Unpublished - 2023

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10.26182/4vp0-6d15

THESIS - DOCTOR OF PHILOSOPHY - BHATTARAI Rudra - 2023
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2 Article

Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat
Bhattarai, R., Liu, H., Siddique, K. H. M. & Yan, G., Dec 2023, In: BMC Plant Biology. 23, 1, 237.
Research output: Contribution to journal › Article › peer-review

Open Access
Characterisation of a 4a QTL for metribuzin resistance in wheat by developing near‐isogenic lines
Bhattarai, R., Liu, H., Siddique, K. H. M. & Yan, G., Sept 2021, In: Plants. 10, 9, 1856.
Research output: Contribution to journal › Article › peer-review

Open Access
1 Citation (Scopus)

Cite this

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title = "Development and characterisation of near-isogenic lines for metribuzin resistance in wheat (Triticumaestivum L.)",

abstract = "Metribuzin resistance in wheat is crucial for successful weed control. Near-isogenic lines (NILs) targeting a major metribuzin resistance locus explaining 69% of the phenotypic variance were developed and studied by mRNA sequencing and proteomic analyses, revealing nitrate excretion transporter, aspartyl protease, glycine-rich protein, WD repeats, AB hydrolase_1 and PsbP as major candidate genes/proteins associated with metribuzin resistance. A number of molecular markers associated with the candidate genes responsible for metribuzin resistance were identified, which can be validated and applied to marker-assisted selection (MAS) in wheat breeding while identified genes/proteins can help to understand genetic mechanism of metribuzin resistance in wheat.",

keywords = "Metribuzin, marker-assisted selection, wheat, near-isogenic lines, alleles, gene, protein and sequencing",

author = "Rudra Bhattarai",

year = "2023",

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language = "English",

school = "The University of Western Australia",

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T1 - Development and characterisation of near-isogenic lines for metribuzin resistance in wheat (Triticumaestivum L.)

AU - Bhattarai, Rudra

PY - 2023

Y1 - 2023

N2 - Metribuzin resistance in wheat is crucial for successful weed control. Near-isogenic lines (NILs) targeting a major metribuzin resistance locus explaining 69% of the phenotypic variance were developed and studied by mRNA sequencing and proteomic analyses, revealing nitrate excretion transporter, aspartyl protease, glycine-rich protein, WD repeats, AB hydrolase_1 and PsbP as major candidate genes/proteins associated with metribuzin resistance. A number of molecular markers associated with the candidate genes responsible for metribuzin resistance were identified, which can be validated and applied to marker-assisted selection (MAS) in wheat breeding while identified genes/proteins can help to understand genetic mechanism of metribuzin resistance in wheat.

AB - Metribuzin resistance in wheat is crucial for successful weed control. Near-isogenic lines (NILs) targeting a major metribuzin resistance locus explaining 69% of the phenotypic variance were developed and studied by mRNA sequencing and proteomic analyses, revealing nitrate excretion transporter, aspartyl protease, glycine-rich protein, WD repeats, AB hydrolase_1 and PsbP as major candidate genes/proteins associated with metribuzin resistance. A number of molecular markers associated with the candidate genes responsible for metribuzin resistance were identified, which can be validated and applied to marker-assisted selection (MAS) in wheat breeding while identified genes/proteins can help to understand genetic mechanism of metribuzin resistance in wheat.

KW - Metribuzin

KW - marker-assisted selection

KW - wheat

KW - near-isogenic lines

KW - alleles

KW - gene

KW - protein and sequencing

U2 - 10.26182/4vp0-6d15

DO - 10.26182/4vp0-6d15

M3 - Doctoral Thesis

ER -

Development and characterisation of near-isogenic lines for metribuzin resistance in wheat (Triticumaestivum L.)

Abstract

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Research output

Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat

Characterisation of a 4a QTL for metribuzin resistance in wheat by developing near‐isogenic lines

Cite this