Development and characterisation of an optimised basic fibroblast growth factor (FGF-2) formulation for the repair of chronic tympanic membrane perforations

Leah Benington

doi:10.26182/dkfq-7s51

Development and characterisation of an optimised basic fibroblast growth factor (FGF-2) formulation for the repair of chronic tympanic membrane perforations

Leah Benington

School of Allied Health

Research output: Thesis › Doctoral Thesis

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Abstract

Chronic tympanic membrane perforations are conventionally treated with expensive surgical procedures. The application of basic fibroblast growth factor (FGF-2) within a supportive scaffold promises a minimally invasive alternative, however, the instability of FGF-2 limited its clinical use. This study demonstrated successful methods for FGF-2 stabilisation that potentiated its cellular proliferative, migratory and wound healing effects in vitro compared with non-stabilised FGF-2. Additionally, alginate-based scaffold products incorporating stabilised FGF-2 demonstrated more favourable cellular interactions and sustained cellular proliferation in vitro than controls, providing a promising basis for their further development into medicinal products for the repair of chronic tympanic membrane perforations.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Lim, Lee Yong, Supervisor Rajan, Gunesh, Supervisor Locher, Connie, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	11 Oct 2020
DOIs	https://doi.org/10.26182/dkfq-7s51
Publication status	Unpublished - 2020

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Embargoed from 05/11/2020 to 22/08/2022. Made publicly available on 22/08/2022.

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10.26182/dkfq-7s51

THESIS - DOCTOR OF PHILOSOPHY - BENINGTON, Leah Renee - 2020 - Part 1
THESIS - DOCTOR OF PHILOSOPHY - BENINGTON, Leah Renee - 2020 - Part 2
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Cite this

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abstract = "Chronic tympanic membrane perforations are conventionally treated with expensive surgical procedures. The application of basic fibroblast growth factor (FGF-2) within a supportive scaffold promises a minimally invasive alternative, however, the instability of FGF-2 limited its clinical use. This study demonstrated successful methods for FGF-2 stabilisation that potentiated its cellular proliferative, migratory and wound healing effects in vitro compared with non-stabilised FGF-2. Additionally, alginate-based scaffold products incorporating stabilised FGF-2 demonstrated more favourable cellular interactions and sustained cellular proliferation in vitro than controls, providing a promising basis for their further development into medicinal products for the repair of chronic tympanic membrane perforations.",

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AB - Chronic tympanic membrane perforations are conventionally treated with expensive surgical procedures. The application of basic fibroblast growth factor (FGF-2) within a supportive scaffold promises a minimally invasive alternative, however, the instability of FGF-2 limited its clinical use. This study demonstrated successful methods for FGF-2 stabilisation that potentiated its cellular proliferative, migratory and wound healing effects in vitro compared with non-stabilised FGF-2. Additionally, alginate-based scaffold products incorporating stabilised FGF-2 demonstrated more favourable cellular interactions and sustained cellular proliferation in vitro than controls, providing a promising basis for their further development into medicinal products for the repair of chronic tympanic membrane perforations.

KW - Fibroblast Growth Factor 2

KW - Scaffold

KW - basic fibroblast growth factor

KW - stabilisation

KW - wound healing

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KW - alginate

U2 - 10.26182/dkfq-7s51

DO - 10.26182/dkfq-7s51

M3 - Doctoral Thesis

ER -

Development and characterisation of an optimised basic fibroblast growth factor (FGF-2) formulation for the repair of chronic tympanic membrane perforations

Abstract

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