Development and application of in vitro models in the investigation of oral carcinogenesis

Ravi Teja Chitturi Suryaprakash

Research output: ThesisDoctoral Thesis

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Abstract

An effective in vitro model of oral cancer is necessary to study carcinogenesis and to test potential chemopreventive agents. The aim of this thesis was to develop such a model using 3-dimensional spheroid cell culture. Normal and dysplastic cells were cultured using traditional and spheroid cell culture methods. They were treated with the carcinogen 4-nitroquinoline 1-oxide, followed by phenotypic & genotypic assessment. Potential targets for chemoprevention were identified using qPCR & RNASeq. The model was then used in a proof of concept study to demonstrate it could be used to assess the efficacy of chemopreventive agents in oral cancer.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Kujan, Omar, Supervisor
  • Shearston, Kate, Supervisor
  • Farah, Camile, Supervisor
  • Fox, Simon, Supervisor
Thesis sponsors
Award date15 Feb 2022
DOIs
Publication statusUnpublished - 2022

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