An effective in vitro model of oral cancer is necessary to study carcinogenesis and to test potential chemopreventive agents. The aim of this thesis was to develop such a model using 3-dimensional spheroid cell culture. Normal and dysplastic cells were cultured using traditional and spheroid cell culture methods. They were treated with the carcinogen 4-nitroquinoline 1-oxide, followed by phenotypic & genotypic assessment. Potential targets for chemoprevention were identified using qPCR & RNASeq. The model was then used in a proof of concept study to demonstrate it could be used to assess the efficacy of chemopreventive agents in oral cancer.
|Qualification||Doctor of Philosophy|
|Award date||15 Feb 2022|
|Publication status||Unpublished - 2022|