Abstract
An effective in vitro model of oral cancer is necessary to study carcinogenesis and to test potential chemopreventive agents. The aim of this thesis was to develop such a model using 3-dimensional spheroid cell culture. Normal and dysplastic cells were cultured using traditional and spheroid cell culture methods. They were treated with the carcinogen 4-nitroquinoline 1-oxide, followed by phenotypic & genotypic assessment. Potential targets for chemoprevention were identified using qPCR & RNASeq. The model was then used in a proof of concept study to demonstrate it could be used to assess the efficacy of chemopreventive agents in oral cancer.
| Original language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution |
|
| Supervisors/Advisors |
|
| Thesis sponsors | |
| Award date | 15 Feb 2022 |
| DOIs | |
| Publication status | Unpublished - 2022 |
Access to Document
- THESIS - DOCTOR OF PHILOSOPHY - CHITTURI SURYAPRAKASH, Ravi Teja - 2022
This work is protected by Copyright. You may print or download ONE copy of this document for the purpose of your own non-commercial research or study. Any other use requires permission from the copyright owner. The Copyright Act requires you to attribute any copyright works you quote or paraphrase.