@inbook{1ebd4bb2c1f94a1a90c7771c556f033c,
title = "Determination of Free Proline in Plants",
abstract = "Proline metabolism has been associated with the induction of reactive oxygen species (ROS), antioxidant enzymes, and the control of cellular redox status. Moreover, proline accumulation is a highly evolutionarily conserved response to diverse abiotic stresses in plants. Thus, proline quantification has been helpful in abiotic stress research as a stress marker. The need for a reliable, fast, and simple method to detect proline in plant tissues is a powerful resource to imply the physiological status of plants under abiotic stress. This chapter summarizes the main strategies for proline extraction and quantification, highlighting their limitations and advantages, and recommends and details a specific protocol for proline extraction and quantification. The chapter provides a friendly version of this protocol with notes useful for researchers to perform the protocol.",
keywords = "Abiotic stress marker, Detection, Extraction, Plants, Proline, ROS, Spectrophotometric methods",
author = "Florencia Sena and Jorge Monza and Santiago Signorelli",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3826-2_12",
language = "English",
volume = "2798",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "183--194",
booktitle = "Methods in Molecular Biology",
address = "United States",
}