Detection of genome-edited and endogenously expressed G protein-coupled receptors

Mark Soave, Leigh A Stoddart, Carl W White, Laura E Kilpatrick, Joëlle Goulding, Stephen J Briddon, Stephen J Hill

Research output: Contribution to journalReview articlepeer-review

7 Citations (Scopus)


G protein-coupled receptors (GPCRs) are the largest family of membrane receptors and major targets for FDA-approved drugs. The ability to quantify GPCR expression and ligand binding characteristics in different cell types and tissues is therefore important for drug discovery. The advent of genome editing along with developments in fluorescent ligand design offers exciting new possibilities to probe GPCRs in their native environment. This review provides an overview of the recent technical advances employed to study the localisation and ligand binding characteristics of genome-edited and endogenously expressed GPCRs.

Original languageEnglish
Pages (from-to)2585-2601
Number of pages17
JournalThe FEBS Journal
Issue number8
Early online date28 Jan 2021
Publication statusPublished - Apr 2021


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