Detection of DNA degradation in porcine dental tissue as a precursor for estimating post-mortem interval

Shruti Gulati

    Research output: ThesisMaster's Thesis

    152 Downloads (Pure)

    Abstract

    The time elapsed after death or PMI is an important component in forensic investigations. Previous researches from many disciplines in forensics have worked on developing methods for accurately establishing PMI. A few studies have also been done to examine DNA degradation over time and to assess any correlation between degradation and time. It has been suggested that DNA within hard tissues such as bones and teeth, being resistant to environmental insults, are suitable tissues for such studies. This study examines the degradation of DNA in porcine dental tissue at different time- points (days 0, 1, 6, 15 and 28) to examine the relationship between degradation and time elapsed since death at 30°C .The methods used to assess the quantity of DNA in this study are NanoDrop spectrometry and real-time PCR and quality of DNA was evaluated by end- point PCR using three primer pairs targeting different amplicon sizes viz. 75 bp, 232 bp and 399 bp. This study also aims to compare dental pulp and powdered teeth as sources of DNA to evaluate DNA degradation. The results of the study give an overall impression of gradual degradation of DNA with time elapsed at 30°C. However, some inconsistencies were present at certain time-points and also among the different tests, particularly with powdered teeth samples. Dental pulp however gave more consistent results. Although no particular pattern was observed in the degradation of DNA at the different time-points, it was established that dental pulp is a better source of DNA for degradation analysis.
    Original languageEnglish
    QualificationMasters
    Publication statusUnpublished - 2012

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