Detection of biological uranium reduction using magnetic resonance

Sarah Vogt, B.D. Stewart, J.D. Seymour, B.M. Peyton, S.L. Codd

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The conversion of soluble uranyl ions (UO22+) by bacterial reduction to sparingly soluble uraninite (UO2(s)) is being studied as a way of immobilizing subsurface uranium contamination. Under anaerobic conditions, several known types of bacteria including iron and sulfate reducing bacteria have been shown to reduce U (VI) to U (IV). Experiments using a suspension of uraninite (UO2(s)) particles produced by Shewanella putrefaciens CN32 bacteria show a dependence of both longitudinal (T1) and transverse (T2) magnetic resonance (MR) relaxation times on the oxidation state and solubility of the uranium. Gradient echo and spin echo MR images were compared to quantify the effect caused by the magnetic field fluctuations ($T_{2}^{*} $) of the uraninite particles and soluble uranyl ions. Since the precipitate studied was suspended in liquid water, the effects of concentration and particle aggregation were explored. A suspension of uraninite particles was injected into a polysaccharide gel, which simulates the precipitation environment of uraninite in the extracellular biofilm matrix. A reduction in the T2 of the gel surrounding the particles was observed. Tests done in situ using three bioreactors under different mixing conditions, continuously stirred, intermittently stirred, and not stirred, showed a quantifiable T2 magnetic relaxation effect over the extent of the reaction. © 2011 Wiley Periodicals, Inc.
    Original languageEnglish
    Pages (from-to)877-883
    JournalBiotechnology and Bioengineering
    Volume109
    Issue number4
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    Uranium
    Magnetic resonance
    Bacteria
    Magnetic Resonance Spectroscopy
    Particles (particulate matter)
    Suspensions
    Shewanella putrefaciens
    Gels
    Ions
    Magnetic relaxation
    Biofilms
    Bioreactors
    Magnetic Fields
    Polysaccharides
    Relaxation time
    Solubility
    Sulfates
    Extracellular Matrix
    Precipitates
    Contamination

    Cite this

    Vogt, Sarah ; Stewart, B.D. ; Seymour, J.D. ; Peyton, B.M. ; Codd, S.L. / Detection of biological uranium reduction using magnetic resonance. In: Biotechnology and Bioengineering. 2012 ; Vol. 109, No. 4. pp. 877-883.
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    abstract = "The conversion of soluble uranyl ions (UO22+) by bacterial reduction to sparingly soluble uraninite (UO2(s)) is being studied as a way of immobilizing subsurface uranium contamination. Under anaerobic conditions, several known types of bacteria including iron and sulfate reducing bacteria have been shown to reduce U (VI) to U (IV). Experiments using a suspension of uraninite (UO2(s)) particles produced by Shewanella putrefaciens CN32 bacteria show a dependence of both longitudinal (T1) and transverse (T2) magnetic resonance (MR) relaxation times on the oxidation state and solubility of the uranium. Gradient echo and spin echo MR images were compared to quantify the effect caused by the magnetic field fluctuations ($T_{2}^{*} $) of the uraninite particles and soluble uranyl ions. Since the precipitate studied was suspended in liquid water, the effects of concentration and particle aggregation were explored. A suspension of uraninite particles was injected into a polysaccharide gel, which simulates the precipitation environment of uraninite in the extracellular biofilm matrix. A reduction in the T2 of the gel surrounding the particles was observed. Tests done in situ using three bioreactors under different mixing conditions, continuously stirred, intermittently stirred, and not stirred, showed a quantifiable T2 magnetic relaxation effect over the extent of the reaction. {\circledC} 2011 Wiley Periodicals, Inc.",
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    Detection of biological uranium reduction using magnetic resonance. / Vogt, Sarah; Stewart, B.D.; Seymour, J.D.; Peyton, B.M.; Codd, S.L.

    In: Biotechnology and Bioengineering, Vol. 109, No. 4, 2012, p. 877-883.

    Research output: Contribution to journalArticle

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    AU - Vogt, Sarah

    AU - Stewart, B.D.

    AU - Seymour, J.D.

    AU - Peyton, B.M.

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