Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells

Lloyd J D'Orsogna; Ellen M W van der Meer-Prins; Yvonne M Zoet; Dave L Roelen; Ilias I N Doxiadis; Frans H J Claas

doi:10.1007/978-1-61779-842-9_19

Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells

Lloyd J D'Orsogna, Ellen M W van der Meer-Prins, Yvonne M Zoet, Dave L Roelen, Ilias I N Doxiadis, Frans H J Claas

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target "detector" cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of "heterologous immunity" from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.

Original language	English
Pages (from-to)	339-349
Number of pages	11
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	882
DOIs	https://doi.org/10.1007/978-1-61779-842-9_19
Publication status	Published - 2012
Externally published	Yes

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10.1007/978-1-61779-842-9_19

Cite this

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title = "Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells",

abstract = "The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target {"}detector{"} cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of {"}heterologous immunity{"} from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.",

keywords = "CD8-Positive T-Lymphocytes/cytology, Clone Cells, Cross Reactions/immunology, HLA Antigens/genetics, Humans, Immunologic Memory/immunology, K562 Cells, Transfection, Viruses/immunology",

author = "D'Orsogna, \{Lloyd J\} and \{van der Meer-Prins\}, \{Ellen M W\} and Zoet, \{Yvonne M\} and Roelen, \{Dave L\} and Doxiadis, \{Ilias I N\} and Claas, \{Frans H J\}",

year = "2012",

doi = "10.1007/978-1-61779-842-9\_19",

language = "English",

volume = "882",

pages = "339--349",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press Inc.",

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TY - JOUR

T1 - Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells

AU - D'Orsogna, Lloyd J

AU - van der Meer-Prins, Ellen M W

AU - Zoet, Yvonne M

AU - Roelen, Dave L

AU - Doxiadis, Ilias I N

AU - Claas, Frans H J

PY - 2012

Y1 - 2012

N2 - The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target "detector" cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of "heterologous immunity" from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.

AB - The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target "detector" cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of "heterologous immunity" from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.

KW - CD8-Positive T-Lymphocytes/cytology

KW - Clone Cells

KW - Cross Reactions/immunology

KW - HLA Antigens/genetics

KW - Humans

KW - Immunologic Memory/immunology

KW - K562 Cells

KW - Transfection

KW - Viruses/immunology

UR - https://www.scopus.com/pages/publications/84867365366

U2 - 10.1007/978-1-61779-842-9_19

DO - 10.1007/978-1-61779-842-9_19

M3 - Article

C2 - 22665243

SN - 1064-3745

VL - 882

SP - 339

EP - 349

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells

Abstract

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