Pentatricopeptide repeat (PPR) proteins are RNA-binding proteins that control organelle gene expression. The thesis aimed to provide insights about RNA recognition by PPR proteins and the possibility of redirecting them to desired targets. I helped show that RPF2 protein could be modified to bind and specifically induce cleavage of nad6 mRNA. I also targeted numerous mitochondrial transcripts by building a library of variant RPF2 proteins. One transformed line showed a reduction in complex V due to specific cleavage of atp1 transcripts induced by the modified RPF2. This thesis contributed to develop specific RNA targeting tools using PPR proteins.
|Qualification||Doctor of Philosophy|
|Award date||17 Jul 2020|
|Publication status||Unpublished - 2020|