Designing PPR proteins to knockdown mitochondrial gene expression

Lilian Vincis Pereira Sanglard

Research output: ThesisDoctoral Thesis

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Pentatricopeptide repeat (PPR) proteins are RNA-binding proteins that control organelle gene expression. The thesis aimed to provide insights about RNA recognition by PPR proteins and the possibility of redirecting them to desired targets. I helped show that RPF2 protein could be modified to bind and specifically induce cleavage of nad6 mRNA. I also targeted numerous mitochondrial transcripts by building a library of variant RPF2 proteins. One transformed line showed a reduction in complex V due to specific cleavage of atp1 transcripts induced by the modified RPF2. This thesis contributed to develop specific RNA targeting tools using PPR proteins.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
  • Colas des Francs-Small, Catherine, Supervisor
  • Small, Ian, Supervisor
  • Lister, Ryan, Supervisor
Award date17 Jul 2020
Publication statusUnpublished - 2020


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