Derivation of two induced pluripotent stem cell lines from a healthy control subject

Dan Zhang; Di Huang; Leon M. Larcher; Khine Zaw; Shang-Chih Chen; Luke Jennings; Tina M. Lamey; Jennifer A. Thompson; Terri L. Mclaren; Fred K. Chen; Samuel Mclenachan

doi:10.1016/j.scr.2024.103621

Derivation of two induced pluripotent stem cell lines from a healthy control subject

Dan Zhang, Di Huang, Leon M. Larcher, Khine Zaw, Shang-Chih Chen, Luke Jennings, Tina M. Lamey, Jennifer A. Thompson, Terri L. Mclaren, Fred K. Chen, Samuel Mclenachan

Centre for Ophthalmology and Visual Science (affiliated with the Lions Eye Institute)

Research output: Contribution to journal › Article › peer-review

Abstract

Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.

Original language	English
Article number	103621
Number of pages	5
Journal	Stem Cell Research
Volume	82
Early online date	5 Dec 2024
DOIs	https://doi.org/10.1016/j.scr.2024.103621
Publication status	Published - Feb 2025

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10.1016/j.scr.2024.103621Licence: CC BY-NC-ND

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abstract = "Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.",

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AU - Zhang, Dan

AU - Huang, Di

AU - Larcher, Leon M.

AU - Zaw, Khine

AU - Chen, Shang-Chih

AU - Jennings, Luke

AU - Lamey, Tina M.

AU - Thompson, Jennifer A.

AU - Mclaren, Terri L.

AU - Chen, Fred K.

AU - Mclenachan, Samuel

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Derivation of two induced pluripotent stem cell lines from a healthy control subject

Abstract

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Accelerating the identification and treatment of splice-altering mutations underlying inherited retinal diseases

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Derivation of two induced pluripotent stem cell lines from a healthy control subject

Abstract

UN SDGs

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Accelerating the identification and treatment of splice-altering mutations underlying inherited retinal diseases

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