Demonstration of Human T Lymphotropic Virus Type I (HTLV-I)-Specific T Cell Responses from Seronegative and Polymerase Chain Reaction-Negative Persons Exposed to HTLV-I

M NISHIMURA, AG KERMODE, M CLERICI, GM SHEARER, JA BERZOFSKY, T UCHIYAMA, SZ WIKTOR, E PATE, BETH MALONEY, A MANNS, W BLATTNER, S JACOBSON

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Abstract

Human T lymphotropic virus type I (HTLV-I) is a human retrovirus etiologically linked to adult T cell leukemia and the progressive chronic neurologic disease HTLV-I-associated myelopathy/tropical spastic paraparesis. Described is a method that measures the production of interleukin-2 from HTLV-I synthetic peptide-stimulated peripheral blood lymphocytes (PBL) of HTLV-I-infected persons. The peptides correspond to immunogenic regions of the HTLV-I Env and Tax proteins. Significantly, this assay demonstrated T cell responses to these HTLV-I peptides from coded PBL samples in 7 of 19 HTLV-I-seronegative polymerase chain reaction-negative persons known to have been exposed to HTLV-I but in none of 16 matched controls without risk factors for exposure (P = .007). The implications of this finding are discussed.

Original languageEnglish
Pages (from-to)334-338
Number of pages5
JournalJournal of Infectious Diseases
Volume170
Issue number2
DOIs
Publication statusPublished - Aug 1994
Externally publishedYes

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