Delayed leaf greening involves a major shift in the expression of cytosolic and mitochondrial ribosomes to plastid ribosomes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)

Toby Bird, Benjamin J. Nestor, Philipp E. Bayer, Guannan Wang, Albina Ilyasova, Clément E. Gille, Bryce E.H. Soraru, Kosala Ranathunge, Anita A. Severn-Ellis, Ricarda Jost, Wolf Rüdiger Scheible, Maheshi Dassanayake, Jacqueline Batley, David Edwards, Hans Lambers, Patrick M. Finnegan

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background and aims: Hakea prostrata (Proteaceae) is a highly phosphorus-use-efficient plant native to southwest Australia. It maintains a high photosynthetic rate at low leaf phosphorus (P) and exhibits delayed leaf greening, a convergent adaptation that increases nutrient-use efficiency. This study aimed to provide broad physiological and gene expression profiles across leaf development, uncovering pathways leading from young leaves as nutrient sinks to mature leaves as low-nutrient, energy-transducing sources. Methods: To explore gene expression underlying delayed greening, we analysed a de novo transcriptome for H. prostrata across five stages of leaf development. Photosynthesis and respiration rates, and foliar pigment, P and nitrogen (N) concentrations were determined, including the division of P into five biochemical fractions. Key results: Transcripts encoding functions associated with leaf structure generally decreased in abundance across leaf development, concomitant with decreases in foliar concentrations of 85% for anthocyanins, 90% for P and 70% for N. The expression of genes associated with photosynthetic function increased during or after leaf expansion, in parallel with increases in photosynthetic pigments and activity, much later in leaf development than in species that do not have delayed greening. As leaves developed, transcript abundance for cytosolic and mitochondrial ribosomal proteins generally declined, whilst transcripts for chloroplast ribosomal proteins increased. Conclusions: There was a much longer temporal separation of leaf cell growth from chloroplast development in H. prostrata than is found in species that lack delayed greening. Transcriptome-guided analysis of leaf development in H. prostrata provided insight into delayed greening as a nutrient-saving strategy in severely phosphorus-impoverished landscapes.

Original languageEnglish
Pages (from-to)7-30
Number of pages24
JournalPlant and Soil
Volume496
Issue number1-2
Early online date27 Sept 2023
DOIs
Publication statusPublished - Mar 2024

Fingerprint

Dive into the research topics of 'Delayed leaf greening involves a major shift in the expression of cytosolic and mitochondrial ribosomes to plastid ribosomes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)'. Together they form a unique fingerprint.

Cite this