Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury

M. Ikegami, T.M. Weaver, J.J. Conkright, Peter Sly, G.F. Ross, J.A. Whitsett, S.W. Glasser

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Abstract

Although the surface properties of surfactant protein (SP)-B and SP-C are similar, the contributions that either protein may make to lung function have not been identified in vivo. Mutations in SP-B cause lethal respiratory failure at birth; however, SP-B null mice are deficient in both SP-B and SP-C. To identify potential contributions of SP-C to lung function in vivo, the following transgenic mice were generated and exposed to 95% O-2 for 3 days: (SP-B+/+,SP-C+/+), (SP-B+/+, SP-C-/-), (SP-B+/-,SP-C+/+), (SP-B+/-,SP-C+/+), and (SP-B+/-,SP-C-/-). Hyperoxia altered pressure-volume curves in mice that were heterozygous for SP- B, and these values were further decreased in (SP-B+/-,SP-C-/-) mice. Likewise, alveolar interleukin (IL)-6 and IL-1beta were maximally increased by O-2 exposure of (SP-B+/-,SP-C-/-) mice compared with the other genotypes. Lung hysteresivity was lower in the (SP-B+/-,SP-C-/-) mice. Surfactant isolated from (SP-B+/+,SP-C-/-) and (SP-B+/-,SP-C-/-) mice failed to stabilize the surface tension of microbubbles, showing that SP-C plays a role in stabilization or recruitment of phospholipid films at low bubble radius. Genetically decreased levels of SP-B combined with superimposed O-2-induced injury reveals the distinct contribution of SP-C to pulmonary function in vivo.
Original languageEnglish
Pages (from-to)519-526
JournalJournal of Applied Physiology
Volume92
Publication statusPublished - 2002

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Ikegami, M., Weaver, T. M., Conkright, J. J., Sly, P., Ross, G. F., Whitsett, J. A., & Glasser, S. W. (2002). Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury. Journal of Applied Physiology, 92, 519-526.