Decreased virulence of cystic fibrosis Pseudomonas aeruginosa in Dictyostelium discoideum

R.S. Bradbury, D.W.E.C. Reid, Tim Inglis, A.C. Champion

    Research output: Contribution to journalArticle

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    Abstract

    The characteristics of clinical and environmental isolates of Pseudomonas aeruginosa from both hospital and community settings were analyzed in a eukaryotic virulence model employing the AX2 and X22 mutants of Dictyostelium discoideum. Thirty-one strains, including two Australian epidemic strains, of P. aeruginosa were analyzed, five from environmental sources, six from clinical sources other than cystic fibrosis (CF) patients and nineteen from CF patients' respiratory secretions. The majority of CF isolates almost uniquely supported the growth of D. discoideum. CF isolates of P. aeruginosa were found to be less virulent than isolates from other sources. Varying degrees of inhibition of the developmental cycle of D. discoideum when growing on CF isolates were also noted. This is the first description of P. aeruginosa isolates from clinical and environmental sources supporting the growth of D. discoideum.
    Original languageEnglish
    Pages (from-to)224-230
    JournalMicrobiology and Immunology
    Volume55
    DOIs
    Publication statusPublished - 2011

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    Dictyostelium
    Cystic Fibrosis
    Pseudomonas aeruginosa
    Virulence
    Growth

    Cite this

    Bradbury, R.S. ; Reid, D.W.E.C. ; Inglis, Tim ; Champion, A.C. / Decreased virulence of cystic fibrosis Pseudomonas aeruginosa in Dictyostelium discoideum. In: Microbiology and Immunology. 2011 ; Vol. 55. pp. 224-230.
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    Decreased virulence of cystic fibrosis Pseudomonas aeruginosa in Dictyostelium discoideum. / Bradbury, R.S.; Reid, D.W.E.C.; Inglis, Tim; Champion, A.C.

    In: Microbiology and Immunology, Vol. 55, 2011, p. 224-230.

    Research output: Contribution to journalArticle

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    AU - Bradbury, R.S.

    AU - Reid, D.W.E.C.

    AU - Inglis, Tim

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    AB - The characteristics of clinical and environmental isolates of Pseudomonas aeruginosa from both hospital and community settings were analyzed in a eukaryotic virulence model employing the AX2 and X22 mutants of Dictyostelium discoideum. Thirty-one strains, including two Australian epidemic strains, of P. aeruginosa were analyzed, five from environmental sources, six from clinical sources other than cystic fibrosis (CF) patients and nineteen from CF patients' respiratory secretions. The majority of CF isolates almost uniquely supported the growth of D. discoideum. CF isolates of P. aeruginosa were found to be less virulent than isolates from other sources. Varying degrees of inhibition of the developmental cycle of D. discoideum when growing on CF isolates were also noted. This is the first description of P. aeruginosa isolates from clinical and environmental sources supporting the growth of D. discoideum.

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