TY - JOUR
T1 - De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds
AU - Dudchenko, Olga
AU - Batra, Sanjit S.
AU - Omer, Arina D.
AU - Nyquist, Sarah K.
AU - Hoeger, Marie
AU - Durand, Neva C.
AU - Shamim, Muhammad S.
AU - Machol, Ido
AU - Lander, Eric S.
AU - Aiden, Aviva Presser
AU - Aiden, Erez Lieberman
PY - 2017/4/7
Y1 - 2017/4/7
N2 - The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. Here we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). We then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors Ae. aegypti and Culex quinquefasciatus, each consisting of three scaffolds corresponding to the three chromosomes in each species. These assemblies indicate that almost all genomic rearrangements among these species occur within, rather than between, chromosome arms. The genome assembly procedure we describe is fast, inexpensive, and accurate, and can be applied to many species.
AB - The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. Here we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). We then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors Ae. aegypti and Culex quinquefasciatus, each consisting of three scaffolds corresponding to the three chromosomes in each species. These assemblies indicate that almost all genomic rearrangements among these species occur within, rather than between, chromosome arms. The genome assembly procedure we describe is fast, inexpensive, and accurate, and can be applied to many species.
UR - http://www.scopus.com/inward/record.url?scp=85017022957&partnerID=8YFLogxK
U2 - 10.1126/science.aal3327
DO - 10.1126/science.aal3327
M3 - Article
C2 - 28336562
AN - SCOPUS:85017022957
VL - 356
SP - 92
EP - 95
JO - Science
JF - Science
SN - 0036-8075
IS - 6333
ER -