Cytological and enzymatic responses to aluminium stress in root tips of Norway spruce seedlings

Nina Elisabeth Nagy, Lars Sandved Dalen, David L. Jones, Berit Swensen, Carl Gunnar Fossdal, Toril D. Eldhuset

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Aluminium (Al) stress reduces plant growth. However, some species such as Norway spruce (Picea abies) seem to tolerate high Al concentrations. The aim of this study was to investigate characteristics possibly involved in Al tolerance in Norway spruce seedlings. Seedlings (10-d-old) were exposed to Al3+ concentrations of 0.5 and 5 mM for up to 168 h. The effect of Al stress on root growth, cell morphology and Al distribution, callose production, and peroxidase and chitinase activity was analysed. Root growth decreased after 1 d and 2 d with 5 and 0.5 mM Al, respectively. Callose concentration increased strongly after 6 h treatment with 5 mM Al. The activity of many peroxidase and chitinase isoforms decreased after 1-24 h exposure of both treatments. Several isoforms increased after 48-168 h exposure to 5 mM Al. We postulate that, with external Al concentrations 0.5 mM or lower, an increased production above constitutive levels of peroxidase or chitinase is not required for Al tolerance in young Norway spruce seedlings. High constitutive levels of peroxidase and chitinase in this species may be part of this Al tolerance.

Original languageEnglish
Pages (from-to)595-607
Number of pages13
JournalNew Phytologist
Volume163
Issue number3
DOIs
Publication statusPublished - 1 Sept 2004
Externally publishedYes

Fingerprint

Dive into the research topics of 'Cytological and enzymatic responses to aluminium stress in root tips of Norway spruce seedlings'. Together they form a unique fingerprint.

Cite this