Cytokine responses in primary and secondary respiratory syncytial virus infections

K. Ugonna, K. Douros, C. D. D. Bingle, Mark L. Everard

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Background: Primary respiratory syncytial virus (RSV) infections are characterized by high levels of IL-8 and an intense neutrophilia. Little is known about the cytokine responses in secondary infections. Preschool children experiencing RSV secondary infections were recruited from the siblings of infants admitted to hospital with RSV acute bronchiolitis.Methods: Fifty-one infants with acute bronchiolitis (39 RSV positive, 12 RSV negative) and 20 age-matched control infants were recruited. In addition, seven older siblings of infants from the RSV-positive cohort and confirmed RSV infection were recruited. Samples of nasal secretions were obtained using a flocked swab, and secretions extracted using centrifugation. Cytokine bead array was used to obtain levels of interleukin (IL)-17A, IL-8, IL-6, IL-21, and tumor necrosis factor-a.Results: Levels of IL-8 and IL-6 were significantly lower in the RSV-positive siblings compared with the RSV-positive infants. There were no significant differences between levels of the other cytokines in the primary and secondary infections.Conclusion: The very high levels of IL-8 and IL-6 response characteristic of the primary RSV infection was not observed in secondary RSV-positive infections and this did not appear to be due to a global reduction in cytokine production.
    Original languageEnglish
    Pages (from-to)946-950
    Number of pages5
    JournalPediatric Research
    Volume79
    Issue number6
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Respiratory Syncytial Virus Infections
    Respiratory Syncytial Viruses
    Cytokines
    Interleukin-8
    Coinfection
    Siblings
    Interleukin-6
    Bronchiolitis
    Interleukin-17
    Preschool Children
    Centrifugation
    Nose
    Tumor Necrosis Factor-alpha

    Cite this

    Ugonna, K. ; Douros, K. ; Bingle, C. D. D. ; Everard, Mark L. / Cytokine responses in primary and secondary respiratory syncytial virus infections. In: Pediatric Research. 2016 ; Vol. 79, No. 6. pp. 946-950.
    @article{be1eaeb5a7f743a5acfcfd7075743691,
    title = "Cytokine responses in primary and secondary respiratory syncytial virus infections",
    abstract = "Background: Primary respiratory syncytial virus (RSV) infections are characterized by high levels of IL-8 and an intense neutrophilia. Little is known about the cytokine responses in secondary infections. Preschool children experiencing RSV secondary infections were recruited from the siblings of infants admitted to hospital with RSV acute bronchiolitis.Methods: Fifty-one infants with acute bronchiolitis (39 RSV positive, 12 RSV negative) and 20 age-matched control infants were recruited. In addition, seven older siblings of infants from the RSV-positive cohort and confirmed RSV infection were recruited. Samples of nasal secretions were obtained using a flocked swab, and secretions extracted using centrifugation. Cytokine bead array was used to obtain levels of interleukin (IL)-17A, IL-8, IL-6, IL-21, and tumor necrosis factor-a.Results: Levels of IL-8 and IL-6 were significantly lower in the RSV-positive siblings compared with the RSV-positive infants. There were no significant differences between levels of the other cytokines in the primary and secondary infections.Conclusion: The very high levels of IL-8 and IL-6 response characteristic of the primary RSV infection was not observed in secondary RSV-positive infections and this did not appear to be due to a global reduction in cytokine production.",
    author = "K. Ugonna and K. Douros and Bingle, {C. D. D.} and Everard, {Mark L.}",
    year = "2016",
    doi = "10.1038/pr.2016.29",
    language = "English",
    volume = "79",
    pages = "946--950",
    journal = "Pediatric Research: international journal of human developmental biology",
    issn = "0031-3998",
    publisher = "INT PEDIATRIC RESEARCH FOUNDATION, INC",
    number = "6",

    }

    Cytokine responses in primary and secondary respiratory syncytial virus infections. / Ugonna, K.; Douros, K.; Bingle, C. D. D.; Everard, Mark L.

    In: Pediatric Research, Vol. 79, No. 6, 2016, p. 946-950.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Cytokine responses in primary and secondary respiratory syncytial virus infections

    AU - Ugonna, K.

    AU - Douros, K.

    AU - Bingle, C. D. D.

    AU - Everard, Mark L.

    PY - 2016

    Y1 - 2016

    N2 - Background: Primary respiratory syncytial virus (RSV) infections are characterized by high levels of IL-8 and an intense neutrophilia. Little is known about the cytokine responses in secondary infections. Preschool children experiencing RSV secondary infections were recruited from the siblings of infants admitted to hospital with RSV acute bronchiolitis.Methods: Fifty-one infants with acute bronchiolitis (39 RSV positive, 12 RSV negative) and 20 age-matched control infants were recruited. In addition, seven older siblings of infants from the RSV-positive cohort and confirmed RSV infection were recruited. Samples of nasal secretions were obtained using a flocked swab, and secretions extracted using centrifugation. Cytokine bead array was used to obtain levels of interleukin (IL)-17A, IL-8, IL-6, IL-21, and tumor necrosis factor-a.Results: Levels of IL-8 and IL-6 were significantly lower in the RSV-positive siblings compared with the RSV-positive infants. There were no significant differences between levels of the other cytokines in the primary and secondary infections.Conclusion: The very high levels of IL-8 and IL-6 response characteristic of the primary RSV infection was not observed in secondary RSV-positive infections and this did not appear to be due to a global reduction in cytokine production.

    AB - Background: Primary respiratory syncytial virus (RSV) infections are characterized by high levels of IL-8 and an intense neutrophilia. Little is known about the cytokine responses in secondary infections. Preschool children experiencing RSV secondary infections were recruited from the siblings of infants admitted to hospital with RSV acute bronchiolitis.Methods: Fifty-one infants with acute bronchiolitis (39 RSV positive, 12 RSV negative) and 20 age-matched control infants were recruited. In addition, seven older siblings of infants from the RSV-positive cohort and confirmed RSV infection were recruited. Samples of nasal secretions were obtained using a flocked swab, and secretions extracted using centrifugation. Cytokine bead array was used to obtain levels of interleukin (IL)-17A, IL-8, IL-6, IL-21, and tumor necrosis factor-a.Results: Levels of IL-8 and IL-6 were significantly lower in the RSV-positive siblings compared with the RSV-positive infants. There were no significant differences between levels of the other cytokines in the primary and secondary infections.Conclusion: The very high levels of IL-8 and IL-6 response characteristic of the primary RSV infection was not observed in secondary RSV-positive infections and this did not appear to be due to a global reduction in cytokine production.

    U2 - 10.1038/pr.2016.29

    DO - 10.1038/pr.2016.29

    M3 - Article

    VL - 79

    SP - 946

    EP - 950

    JO - Pediatric Research: international journal of human developmental biology

    JF - Pediatric Research: international journal of human developmental biology

    SN - 0031-3998

    IS - 6

    ER -