TY - JOUR
T1 - Cytokine-induced coordinate expression of enzymes of prostaglandin biosynthesis and metabolism: 15-hydroxyprostaglandin dehydrogenase
AU - Mitchell, MD
AU - Goodwin, V
AU - Mesnage, S
AU - Keelan, Jeffrey
PY - 2000
Y1 - 2000
N2 - The effects of pro-inflammatory cytokines on 15-hydroxyprostaglandin dehydrogenase (PGDH) expression and prostaglandin (PG) metabolizing activity were investigated in placental tissue. Treatment of trophoblast cells in primary culture with interleukin-1 beta (IL-1 beta) or tumour necrosis factor-alpha (TNF-alpha) resulted in decreased prostaglandin metabolizing activity. This was assessed both by determining the ratio of production of prostaglandins to their metabolites, and by Northern blot analysis of PGDH mRNA abundance. Dexamethasone had similar inhibitory effect. We hypothesize that proinflammatory cytokines act to enhance prostaglandin actions through coordinated effects on activities of enzymes of both prostaglandin biosynthesis and metabolism, perhaps in concert with effects on receptor function. (C) 2000 Harcourt Publishers Ltd.
AB - The effects of pro-inflammatory cytokines on 15-hydroxyprostaglandin dehydrogenase (PGDH) expression and prostaglandin (PG) metabolizing activity were investigated in placental tissue. Treatment of trophoblast cells in primary culture with interleukin-1 beta (IL-1 beta) or tumour necrosis factor-alpha (TNF-alpha) resulted in decreased prostaglandin metabolizing activity. This was assessed both by determining the ratio of production of prostaglandins to their metabolites, and by Northern blot analysis of PGDH mRNA abundance. Dexamethasone had similar inhibitory effect. We hypothesize that proinflammatory cytokines act to enhance prostaglandin actions through coordinated effects on activities of enzymes of both prostaglandin biosynthesis and metabolism, perhaps in concert with effects on receptor function. (C) 2000 Harcourt Publishers Ltd.
UR - https://www.scopus.com/pages/publications/0034030447
U2 - 10.1054/plef.1999.0117
DO - 10.1054/plef.1999.0117
M3 - Article
C2 - 10765972
SN - 0952-3278
VL - 62
SP - 1
EP - 5
JO - Prostaglandins, Leukotrienes and Essential Fatty Acids
JF - Prostaglandins, Leukotrienes and Essential Fatty Acids
ER -