Customizing the shape and microenvironment biochemistry of biocompatible macroscopic plant-derived cellulose scaffolds

Ryan J. Hickey, Daniel J. Modulevsky, Charles M. Cuerrier, Andrew E. Pelling

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Plant-derived cellulose scaffolds constitute a highly viable and interesting biomaterial. They retain a high flexibility in shape and structure, present the ability to tune surface biochemistry, display a high degree of biocompatibility, exhibit vascularization, and are widely available and easily produced. What is also immediately clear is that pre-existing cellulose structures in plants can also provide candidates for specific tissue engineering applications. Here, we report a new preparation and fabrication approach for producing large scale scaffolds with customizable macroscopic structures that support cell attachment and invasion both in vitro and in vivo. This new fabrication method significantly improves cell attachment compared to that in our previous work. Moreover, the materials remain highly biocompatible and retain vascularization properties in vivo. We present proof-of-concept studies that demonstrate how hydrogels can be temporarily or permanently cast onto the macroscopic scaffolds to create composite plant-derived cellulose biomaterials. This inverse molding approach allows us to provide temporary or permanent biochemical cues to invading cells in vitro. The development of a new-generation of rapidly and efficiently produced composite plant-derived biomaterials provides an important proof that such biomaterials have the potential for numerous applications in tissue engineering.

Original languageEnglish
Pages (from-to)3726-3736
Number of pages21
JournalACS Biomaterials Science and Engineering
Volume4
Issue number11
DOIs
Publication statusPublished - Nov 2018

Cite this

@article{15387a383eb54bd89c29b95a7e73e45f,
title = "Customizing the shape and microenvironment biochemistry of biocompatible macroscopic plant-derived cellulose scaffolds",
abstract = "Plant-derived cellulose scaffolds constitute a highly viable and interesting biomaterial. They retain a high flexibility in shape and structure, present the ability to tune surface biochemistry, display a high degree of biocompatibility, exhibit vascularization, and are widely available and easily produced. What is also immediately clear is that pre-existing cellulose structures in plants can also provide candidates for specific tissue engineering applications. Here, we report a new preparation and fabrication approach for producing large scale scaffolds with customizable macroscopic structures that support cell attachment and invasion both in vitro and in vivo. This new fabrication method significantly improves cell attachment compared to that in our previous work. Moreover, the materials remain highly biocompatible and retain vascularization properties in vivo. We present proof-of-concept studies that demonstrate how hydrogels can be temporarily or permanently cast onto the macroscopic scaffolds to create composite plant-derived cellulose biomaterials. This inverse molding approach allows us to provide temporary or permanent biochemical cues to invading cells in vitro. The development of a new-generation of rapidly and efficiently produced composite plant-derived biomaterials provides an important proof that such biomaterials have the potential for numerous applications in tissue engineering.",
keywords = "biomaterials, cellulose, plants, scaffolds, biocompatibility, angiogenesis, 3D CELL-CULTURE, BACTERIAL CELLULOSE, STROMAL CELLS, HYDROGELS, CARTILAGE, COMPOSITE, NANOCELLULOSE, PROLIFERATION, REPLACEMENT, STIFFNESS",
author = "Hickey, {Ryan J.} and Modulevsky, {Daniel J.} and Cuerrier, {Charles M.} and Pelling, {Andrew E.}",
year = "2018",
month = "11",
doi = "10.1021/acsbiomaterials.8b00178",
language = "English",
volume = "4",
pages = "3726--3736",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "11",

}

Customizing the shape and microenvironment biochemistry of biocompatible macroscopic plant-derived cellulose scaffolds. / Hickey, Ryan J.; Modulevsky, Daniel J.; Cuerrier, Charles M.; Pelling, Andrew E.

In: ACS Biomaterials Science and Engineering, Vol. 4, No. 11, 11.2018, p. 3726-3736.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Customizing the shape and microenvironment biochemistry of biocompatible macroscopic plant-derived cellulose scaffolds

AU - Hickey, Ryan J.

AU - Modulevsky, Daniel J.

AU - Cuerrier, Charles M.

AU - Pelling, Andrew E.

PY - 2018/11

Y1 - 2018/11

N2 - Plant-derived cellulose scaffolds constitute a highly viable and interesting biomaterial. They retain a high flexibility in shape and structure, present the ability to tune surface biochemistry, display a high degree of biocompatibility, exhibit vascularization, and are widely available and easily produced. What is also immediately clear is that pre-existing cellulose structures in plants can also provide candidates for specific tissue engineering applications. Here, we report a new preparation and fabrication approach for producing large scale scaffolds with customizable macroscopic structures that support cell attachment and invasion both in vitro and in vivo. This new fabrication method significantly improves cell attachment compared to that in our previous work. Moreover, the materials remain highly biocompatible and retain vascularization properties in vivo. We present proof-of-concept studies that demonstrate how hydrogels can be temporarily or permanently cast onto the macroscopic scaffolds to create composite plant-derived cellulose biomaterials. This inverse molding approach allows us to provide temporary or permanent biochemical cues to invading cells in vitro. The development of a new-generation of rapidly and efficiently produced composite plant-derived biomaterials provides an important proof that such biomaterials have the potential for numerous applications in tissue engineering.

AB - Plant-derived cellulose scaffolds constitute a highly viable and interesting biomaterial. They retain a high flexibility in shape and structure, present the ability to tune surface biochemistry, display a high degree of biocompatibility, exhibit vascularization, and are widely available and easily produced. What is also immediately clear is that pre-existing cellulose structures in plants can also provide candidates for specific tissue engineering applications. Here, we report a new preparation and fabrication approach for producing large scale scaffolds with customizable macroscopic structures that support cell attachment and invasion both in vitro and in vivo. This new fabrication method significantly improves cell attachment compared to that in our previous work. Moreover, the materials remain highly biocompatible and retain vascularization properties in vivo. We present proof-of-concept studies that demonstrate how hydrogels can be temporarily or permanently cast onto the macroscopic scaffolds to create composite plant-derived cellulose biomaterials. This inverse molding approach allows us to provide temporary or permanent biochemical cues to invading cells in vitro. The development of a new-generation of rapidly and efficiently produced composite plant-derived biomaterials provides an important proof that such biomaterials have the potential for numerous applications in tissue engineering.

KW - biomaterials

KW - cellulose

KW - plants

KW - scaffolds

KW - biocompatibility

KW - angiogenesis

KW - 3D CELL-CULTURE

KW - BACTERIAL CELLULOSE

KW - STROMAL CELLS

KW - HYDROGELS

KW - CARTILAGE

KW - COMPOSITE

KW - NANOCELLULOSE

KW - PROLIFERATION

KW - REPLACEMENT

KW - STIFFNESS

U2 - 10.1021/acsbiomaterials.8b00178

DO - 10.1021/acsbiomaterials.8b00178

M3 - Article

VL - 4

SP - 3726

EP - 3736

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 11

ER -