Culture and characterization of smooth muscle cells from porcine retinal microvessels

B-C. Mortimer, Dao-Yi Yu, William Morgan, V.A. Alder

Research output: Contribution to journalArticle

Abstract

Purpose: To establish long-term cultures of retinal vascular smooth muscle cells for future studies of their functions under normal and diseased conditions.Methods: Smooth muscle cells (SMC) from porcine retinal microvessels were isolated and cultured.Results: Cells in culture retained the common biochemical characteristics of SMC propagated from other sources. The cells were large, polygonal, spindle shaped and demonstrated the presence of smooth muscle-specific alpha-actin. Post-confluent cultures showed the 'hill and valley' multilayer growth characteristics.However, the growth rate was lower and the population doubling time was significantly larger than those reported for SMC propagated from big vessels.Conclusion: Retinal vascular SMC could be cultured separately from other cell types.The availability of this culture provides a unique model for functional and metabolic studies of the retinal vessels.
Original languageEnglish
Pages (from-to)S80-S83
JournalAustralian and New Zealand Journal of Ophthalmology
Volume26
Issue numberSupplement
DOIs
Publication statusPublished - 1998

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