Cryptosporidium homai n. sp. (Apicomplexa Cryptosporidiiae) from the guinea pig (Cavia porcellus)

Alireza Zahedi, Zoey Durmic, Alexander W. Gofton, Susan Kueh, Jill Austen, Malcolm Lawson, Lauren Callahan, John Jardine, Una Ryan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The morphological, biological, and molecular characterisation of a new Cryptosporidium species from the guinea pig (Cavia porcellus) are described, and the species name Cryptosporidium homai n. sp. is proposed. Histological analysis conducted on a post-mortem sample from a guinea pig euthanised due to respiratory distress, identified developmental stages of C. homai n. sp. (trophozoites and meronts) along the intestinal epithelium. Molecular analysis at 18S rRNA (18S), actin and hsp70 loci was then conducted on faeces from an additional 7 guinea pigs positive for C. homai n. sp. At the 18S, actin and hsp70 loci, C. homai n. sp. exhibited genetic distances ranging from 3.1% to 14.3%, 14.4% to 24.5%, and 6.6% to 20.9% from other Cryptosporidium spp., respectively. At the 18S locus, C. homai n. sp. shared 99.1% similarity with a previously described Cryptosporidium genotype in guinea pigs from Brazil and it is likely that they are the same species, however this cannot be confirmed as actin and hsp70 sequences from the Brazilian guinea pig genotype are not available. Phylogenetic analysis of concatenated 18S, actin and hsp70 sequences showed that C. homai n. sp. exhibited 9.1% to 17.3% genetic distance from all other Cryptosporidium spp. This clearly supports the validity of C. homai n. sp. as a separate species.

Original languageEnglish
Pages (from-to)92-101
Number of pages10
JournalVeterinary Parasitology
Volume245
DOIs
Publication statusPublished - 15 Oct 2017

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Apicomplexa
Cryptosporidium
guinea pigs
Guinea Pigs
actin
Actins
loci
genetic distance
Genotype
meronts
Trophozoites
trophozoites
genotype
Intestinal Mucosa
intestinal mucosa
distress
Feces
Names
Brazil
Miozoa

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Zahedi, Alireza ; Durmic, Zoey ; Gofton, Alexander W. ; Kueh, Susan ; Austen, Jill ; Lawson, Malcolm ; Callahan, Lauren ; Jardine, John ; Ryan, Una. / Cryptosporidium homai n. sp. (Apicomplexa Cryptosporidiiae) from the guinea pig (Cavia porcellus). In: Veterinary Parasitology. 2017 ; Vol. 245. pp. 92-101.
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abstract = "The morphological, biological, and molecular characterisation of a new Cryptosporidium species from the guinea pig (Cavia porcellus) are described, and the species name Cryptosporidium homai n. sp. is proposed. Histological analysis conducted on a post-mortem sample from a guinea pig euthanised due to respiratory distress, identified developmental stages of C. homai n. sp. (trophozoites and meronts) along the intestinal epithelium. Molecular analysis at 18S rRNA (18S), actin and hsp70 loci was then conducted on faeces from an additional 7 guinea pigs positive for C. homai n. sp. At the 18S, actin and hsp70 loci, C. homai n. sp. exhibited genetic distances ranging from 3.1{\%} to 14.3{\%}, 14.4{\%} to 24.5{\%}, and 6.6{\%} to 20.9{\%} from other Cryptosporidium spp., respectively. At the 18S locus, C. homai n. sp. shared 99.1{\%} similarity with a previously described Cryptosporidium genotype in guinea pigs from Brazil and it is likely that they are the same species, however this cannot be confirmed as actin and hsp70 sequences from the Brazilian guinea pig genotype are not available. Phylogenetic analysis of concatenated 18S, actin and hsp70 sequences showed that C. homai n. sp. exhibited 9.1{\%} to 17.3{\%} genetic distance from all other Cryptosporidium spp. This clearly supports the validity of C. homai n. sp. as a separate species.",
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Cryptosporidium homai n. sp. (Apicomplexa Cryptosporidiiae) from the guinea pig (Cavia porcellus). / Zahedi, Alireza; Durmic, Zoey; Gofton, Alexander W.; Kueh, Susan; Austen, Jill; Lawson, Malcolm; Callahan, Lauren; Jardine, John; Ryan, Una.

In: Veterinary Parasitology, Vol. 245, 15.10.2017, p. 92-101.

Research output: Contribution to journalArticle

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T1 - Cryptosporidium homai n. sp. (Apicomplexa Cryptosporidiiae) from the guinea pig (Cavia porcellus)

AU - Zahedi, Alireza

AU - Durmic, Zoey

AU - Gofton, Alexander W.

AU - Kueh, Susan

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AU - Jardine, John

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N2 - The morphological, biological, and molecular characterisation of a new Cryptosporidium species from the guinea pig (Cavia porcellus) are described, and the species name Cryptosporidium homai n. sp. is proposed. Histological analysis conducted on a post-mortem sample from a guinea pig euthanised due to respiratory distress, identified developmental stages of C. homai n. sp. (trophozoites and meronts) along the intestinal epithelium. Molecular analysis at 18S rRNA (18S), actin and hsp70 loci was then conducted on faeces from an additional 7 guinea pigs positive for C. homai n. sp. At the 18S, actin and hsp70 loci, C. homai n. sp. exhibited genetic distances ranging from 3.1% to 14.3%, 14.4% to 24.5%, and 6.6% to 20.9% from other Cryptosporidium spp., respectively. At the 18S locus, C. homai n. sp. shared 99.1% similarity with a previously described Cryptosporidium genotype in guinea pigs from Brazil and it is likely that they are the same species, however this cannot be confirmed as actin and hsp70 sequences from the Brazilian guinea pig genotype are not available. Phylogenetic analysis of concatenated 18S, actin and hsp70 sequences showed that C. homai n. sp. exhibited 9.1% to 17.3% genetic distance from all other Cryptosporidium spp. This clearly supports the validity of C. homai n. sp. as a separate species.

AB - The morphological, biological, and molecular characterisation of a new Cryptosporidium species from the guinea pig (Cavia porcellus) are described, and the species name Cryptosporidium homai n. sp. is proposed. Histological analysis conducted on a post-mortem sample from a guinea pig euthanised due to respiratory distress, identified developmental stages of C. homai n. sp. (trophozoites and meronts) along the intestinal epithelium. Molecular analysis at 18S rRNA (18S), actin and hsp70 loci was then conducted on faeces from an additional 7 guinea pigs positive for C. homai n. sp. At the 18S, actin and hsp70 loci, C. homai n. sp. exhibited genetic distances ranging from 3.1% to 14.3%, 14.4% to 24.5%, and 6.6% to 20.9% from other Cryptosporidium spp., respectively. At the 18S locus, C. homai n. sp. shared 99.1% similarity with a previously described Cryptosporidium genotype in guinea pigs from Brazil and it is likely that they are the same species, however this cannot be confirmed as actin and hsp70 sequences from the Brazilian guinea pig genotype are not available. Phylogenetic analysis of concatenated 18S, actin and hsp70 sequences showed that C. homai n. sp. exhibited 9.1% to 17.3% genetic distance from all other Cryptosporidium spp. This clearly supports the validity of C. homai n. sp. as a separate species.

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