Cross-sectional associations of sex hormones with leucocyte telomere length, a marker of biological age, in a community-based cohort of older men

Bu B Yeap, Jennie Hui, Matthew W Knuiman, David J Handelsman, Leon Flicker, Mark L Divitini, Gillian M Arscott, Susan V McLennan, Stephen M Twigg, Osvaldo P Almeida, Graeme J Hankey, Jonathan Golledge, Paul E Norman, John P Beilby

Research output: Contribution to journalArticle

Abstract

CONTEXT: Telomeres protect chromosomes from damage, and shorter leucocyte telomere length (LTL) is a marker of advancing biological age. The association between testosterone (T) and its bioactive metabolites, dihydrotestosterone (DHT) and estradiol (E2) with telomere length, particularly in older men, is uncertain. The study aimed to clarify associations of sex hormones with LTL in older men.

PARTICIPANTS AND METHODS: We used cross-sectional data from 2,913 men aged 76.7±3.2 years with morning blood samples assayed for T, DHT, E2 (mass spectrometry), and sex hormone-binding globulin (SHBG, immunoassay), to correlate sex hormones with LTL measured using PCR and expressed as T/S ratio in multivariable linear regression models adjusted for age, cardiometabolic risk factors and cardiovascular disease history.

RESULTS: Average difference per decade of age was T -0.46 nmol/L, DHT -0.11 nmol/L, E2 -7.5 pmol/L, SHBG +10.2 nmol/L, and LTL (T/S ratio) -0.065. E2 correlated with T/S ratio (r=0.038, p=0.039) and SHBG was inversely correlated (r=-0.053, p=0.004). After multivariable adjustment, E2 was associated with T/S ratio (per 1SD increase E2: coefficient 0.011, p=0.043), T and DHT were not associated. When E2 and SHBG were simultaneously included, E2 remained positively (coefficient 0.014, p=0.014) and SHBG inversely (coefficient -0.013, p=0.037) associated with T/S ratio.

CONCLUSIONS: In older men, neither T nor DHT are associated with LTL while E2 is independently associated with LTL and SHBG is inversely associated, thus relating sex hormone exposure to lower biological age. Further research is needed to determine causality and clarify the role of sex hormones in male ageing. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)562-569
JournalClinical Endocrinology
Volume90
Issue number4
Early online date20 Jan 2019
DOIs
Publication statusPublished - Apr 2019

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Telomere
Gonadal Steroid Hormones
Leukocytes
Biomarkers
Dihydrotestosterone
Linear Models
Sex Hormone-Binding Globulin
Immunoassay
Causality
Testosterone
Estradiol
Mass Spectrometry
Cardiovascular Diseases
Chromosomes
Polymerase Chain Reaction
Research

Cite this

@article{77884c7ec06b4c71bd5879c6b7ce857b,
title = "Cross-sectional associations of sex hormones with leucocyte telomere length, a marker of biological age, in a community-based cohort of older men",
abstract = "CONTEXT: Telomeres protect chromosomes from damage, and shorter leucocyte telomere length (LTL) is a marker of advancing biological age. The association between testosterone (T) and its bioactive metabolites, dihydrotestosterone (DHT) and estradiol (E2) with telomere length, particularly in older men, is uncertain. The study aimed to clarify associations of sex hormones with LTL in older men.PARTICIPANTS AND METHODS: We used cross-sectional data from 2,913 men aged 76.7±3.2 years with morning blood samples assayed for T, DHT, E2 (mass spectrometry), and sex hormone-binding globulin (SHBG, immunoassay), to correlate sex hormones with LTL measured using PCR and expressed as T/S ratio in multivariable linear regression models adjusted for age, cardiometabolic risk factors and cardiovascular disease history.RESULTS: Average difference per decade of age was T -0.46 nmol/L, DHT -0.11 nmol/L, E2 -7.5 pmol/L, SHBG +10.2 nmol/L, and LTL (T/S ratio) -0.065. E2 correlated with T/S ratio (r=0.038, p=0.039) and SHBG was inversely correlated (r=-0.053, p=0.004). After multivariable adjustment, E2 was associated with T/S ratio (per 1SD increase E2: coefficient 0.011, p=0.043), T and DHT were not associated. When E2 and SHBG were simultaneously included, E2 remained positively (coefficient 0.014, p=0.014) and SHBG inversely (coefficient -0.013, p=0.037) associated with T/S ratio.CONCLUSIONS: In older men, neither T nor DHT are associated with LTL while E2 is independently associated with LTL and SHBG is inversely associated, thus relating sex hormone exposure to lower biological age. Further research is needed to determine causality and clarify the role of sex hormones in male ageing. This article is protected by copyright. All rights reserved.",
author = "Yeap, {Bu B} and Jennie Hui and Knuiman, {Matthew W} and Handelsman, {David J} and Leon Flicker and Divitini, {Mark L} and Arscott, {Gillian M} and McLennan, {Susan V} and Twigg, {Stephen M} and Almeida, {Osvaldo P} and Hankey, {Graeme J} and Jonathan Golledge and Norman, {Paul E} and Beilby, {John P}",
year = "2019",
month = "4",
doi = "10.1111/cen.13918",
language = "English",
volume = "90",
pages = "562--569",
journal = "Clinical Endocrinology",
issn = "0300-0664",
publisher = "John Wiley & Sons",
number = "4",

}

TY - JOUR

T1 - Cross-sectional associations of sex hormones with leucocyte telomere length, a marker of biological age, in a community-based cohort of older men

AU - Yeap, Bu B

AU - Hui, Jennie

AU - Knuiman, Matthew W

AU - Handelsman, David J

AU - Flicker, Leon

AU - Divitini, Mark L

AU - Arscott, Gillian M

AU - McLennan, Susan V

AU - Twigg, Stephen M

AU - Almeida, Osvaldo P

AU - Hankey, Graeme J

AU - Golledge, Jonathan

AU - Norman, Paul E

AU - Beilby, John P

PY - 2019/4

Y1 - 2019/4

N2 - CONTEXT: Telomeres protect chromosomes from damage, and shorter leucocyte telomere length (LTL) is a marker of advancing biological age. The association between testosterone (T) and its bioactive metabolites, dihydrotestosterone (DHT) and estradiol (E2) with telomere length, particularly in older men, is uncertain. The study aimed to clarify associations of sex hormones with LTL in older men.PARTICIPANTS AND METHODS: We used cross-sectional data from 2,913 men aged 76.7±3.2 years with morning blood samples assayed for T, DHT, E2 (mass spectrometry), and sex hormone-binding globulin (SHBG, immunoassay), to correlate sex hormones with LTL measured using PCR and expressed as T/S ratio in multivariable linear regression models adjusted for age, cardiometabolic risk factors and cardiovascular disease history.RESULTS: Average difference per decade of age was T -0.46 nmol/L, DHT -0.11 nmol/L, E2 -7.5 pmol/L, SHBG +10.2 nmol/L, and LTL (T/S ratio) -0.065. E2 correlated with T/S ratio (r=0.038, p=0.039) and SHBG was inversely correlated (r=-0.053, p=0.004). After multivariable adjustment, E2 was associated with T/S ratio (per 1SD increase E2: coefficient 0.011, p=0.043), T and DHT were not associated. When E2 and SHBG were simultaneously included, E2 remained positively (coefficient 0.014, p=0.014) and SHBG inversely (coefficient -0.013, p=0.037) associated with T/S ratio.CONCLUSIONS: In older men, neither T nor DHT are associated with LTL while E2 is independently associated with LTL and SHBG is inversely associated, thus relating sex hormone exposure to lower biological age. Further research is needed to determine causality and clarify the role of sex hormones in male ageing. This article is protected by copyright. All rights reserved.

AB - CONTEXT: Telomeres protect chromosomes from damage, and shorter leucocyte telomere length (LTL) is a marker of advancing biological age. The association between testosterone (T) and its bioactive metabolites, dihydrotestosterone (DHT) and estradiol (E2) with telomere length, particularly in older men, is uncertain. The study aimed to clarify associations of sex hormones with LTL in older men.PARTICIPANTS AND METHODS: We used cross-sectional data from 2,913 men aged 76.7±3.2 years with morning blood samples assayed for T, DHT, E2 (mass spectrometry), and sex hormone-binding globulin (SHBG, immunoassay), to correlate sex hormones with LTL measured using PCR and expressed as T/S ratio in multivariable linear regression models adjusted for age, cardiometabolic risk factors and cardiovascular disease history.RESULTS: Average difference per decade of age was T -0.46 nmol/L, DHT -0.11 nmol/L, E2 -7.5 pmol/L, SHBG +10.2 nmol/L, and LTL (T/S ratio) -0.065. E2 correlated with T/S ratio (r=0.038, p=0.039) and SHBG was inversely correlated (r=-0.053, p=0.004). After multivariable adjustment, E2 was associated with T/S ratio (per 1SD increase E2: coefficient 0.011, p=0.043), T and DHT were not associated. When E2 and SHBG were simultaneously included, E2 remained positively (coefficient 0.014, p=0.014) and SHBG inversely (coefficient -0.013, p=0.037) associated with T/S ratio.CONCLUSIONS: In older men, neither T nor DHT are associated with LTL while E2 is independently associated with LTL and SHBG is inversely associated, thus relating sex hormone exposure to lower biological age. Further research is needed to determine causality and clarify the role of sex hormones in male ageing. This article is protected by copyright. All rights reserved.

U2 - 10.1111/cen.13918

DO - 10.1111/cen.13918

M3 - Article

VL - 90

SP - 562

EP - 569

JO - Clinical Endocrinology

JF - Clinical Endocrinology

SN - 0300-0664

IS - 4

ER -